« Previous AbstractDevelopment of a smartphone-based real time cost-effective VOC sensor    Next AbstractIn Operando Spectroscopic Ellipsometry Investigation of MOF Thin Films for the Selective Capture of Acetic Acid »

Proc Natl Acad Sci U S A

Title:		"Mot1 activates and represses transcription by direct, ATPase-dependent mechanisms"
Author(s):		Dasgupta A; Darst RP; Martin KJ; Afshari CA; Auble DT;
Address:		"Department of Biochemistry and Molecular Genetics, University of Virginia Health System, 1300 Jefferson Park Avenue, Room 6213, Charlottesville, VA 22908-0733, USA"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2002
Volume:		99
Issue:		5
Page Number:		2666 - 2671
DOI:		10.1073/pnas.052397899
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"Mot1 is an essential yeast Snf2/Swi2-related ATPase that exerts both positive and negative effects on gene expression. In vitro, Mot1 can disrupt TATA-binding protein-DNA complexes in an ATP-dependent reaction. This activity can explain Mot1-mediated transcriptional repression, but how Mot1 activates transcription is unknown. We demonstrate that, remarkably, Mot1 is localized in vivo to promoters for both Mot1-repressed and Mot1-activated genes. Moreover, Mot1 ATPase activity is required for both activation and repression of gene activity. These findings suggest a novel function for the Mot1 ATPase at activated genes, perhaps involving ATP-driven reorganization of the preinitiation complex. Mot1 regulates the expression of approximately 3% of yeast genes in cells grown in rich medium. Most of these genes are repressed by Mot1, consistent with Mot1's ATP-dependent TATA-binding protein-DNA dissociating activity. Additionally, approximately 77% of the Mot1-repressed genes are involved in the diauxic shift, stress response, mating, or sporulation. The gene sets controlled by NC2 and Srb10 are strongly correlated with the Mot1-controlled set, suggesting that these factors cooperate in transcriptional control on a global scale"
Keywords:		"Adenosine Triphosphatases/*metabolism Chromatin/metabolism DNA Helicases/*metabolism Fungal Proteins/*metabolism *Gene Expression Regulation, Fungal Genes, Fungal Oligonucleotide Array Sequence Analysis Repressor Proteins/*metabolism Saccharomyces cerevis;"
Notes:		"MedlineDasgupta, Arindam Darst, Russell P Martin, Karla J Afshari, Cynthia A Auble, David T eng R01 GM055763/GM/NIGMS NIH HHS/ GM55763/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 2002/03/07 Proc Natl Acad Sci U S A. 2002 Mar 5; 99(5):2666-71. doi: 10.1073/pnas.052397899"