| Title: | Three Chemosensory Proteins Involved in Chemoreception of Oedaleus asiaticus (Orthopera: Acridoidea) |
| Author(s): | Zhou YT; Li L; Zhou XR; Tan Y; Pang BP; |
| Address: | "Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, 010020, China. College of Agriculture and Animal Husbandry, Qinghai University, Xining, 810016, China. Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, 010020, China. pangbp@imau.edu.cn" |
| DOI: | 10.1007/s10886-019-01138-5 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "Chemosensory proteins (CSPs) are thought to play roles in the insect olfactory system by binding and carrying hydrophobic odorants across the aqueous sensillar lymph. The band-winged grasshopper, Oedaleus asiaticus Bei-Bienko, is one of the most important grasshopper pests in northern China, but there is little information about its olfactory system. In order to investigate the olfactory functions of CSPs in this pest, three CSP genes (OasiCSP4, OasiCSP11 and OasiCSP12) were expressed in Escherichia coli, and the binding affinities of the three recombinant CSP proteins were measured for 16 volatiles from the host plant (Stipa krylovii), fecal material and body of live adult O. asiaticus using fluorescence competitive binding assays. To further verify their olfactory functions, RNA interference (RNAi) and electrophysiological recording were conducted. The three recombinant proteins displayed different degrees of binding to various volatiles in ligand-binding assays, with OasiCSP12 having higher binding affinities for more volatiles than OasiCSP4 and OasiCSP11. OasiCSP12 exhibited strong binding affinities (Ki < 20 muMu) for five host plant volatiles and one volatile from the live body of adult O. asiaticus. The transcript levels of the three OasiCSP genes were significantly lower after silencing the individual genes by RNAi, which in turn reduced the EAG responses in adults of both sexes to most tested compounds. Our study indicates that these three OasiCSPs are involved in the detection of volatile semiochemicals, and may play important roles in finding host plants and in aggregation in O. asiaticus" |
| Keywords: | "Animals Binding, Competitive Female Grasshoppers/*metabolism Insect Proteins/antagonists & inhibitors/genetics/*metabolism Male Poaceae/chemistry/metabolism Protein Binding RNA Interference RNA, Double-Stranded/metabolism Receptors, Odorant/antagonists &;" |
| Notes: | "MedlineZhou, Yuan-Tao Li, Ling Zhou, Xiao-Rong Tan, Yao Pang, Bao-Ping eng 31760517/National Natural Science Foundation of China/ 2019/12/20 J Chem Ecol. 2020 Feb; 46(2):138-149. doi: 10.1007/s10886-019-01138-5. Epub 2019 Dec 19" |
