| Title: | Pyrolytic profiling nitrosamine specific chemiluminescence detection combined with multivariate chemometric discrimination for non-targeted detection and classification of nitroso compounds in complex samples |
| Address: | "Research Institute of Brewing and Malting, PLC, Lipova 15, 120 44, Prague 2, Czech Republic; Charles University, Department of Analytical Chemistry, Albertov 6, 128 43, Prague 2, Czech Republic. Electronic address: tomas.vrzal@beerresearch.cz. Research Institute of Brewing and Malting, PLC, Lipova 15, 120 44, Prague 2, Czech Republic" |
| DOI: | 10.1016/j.aca.2019.01.033 |
| ISSN/ISBN: | 1873-4324 (Electronic) 0003-2670 (Linking) |
| Abstract: | "The problems of contamination of many products by nitroso compounds have been discussed since 1970's and have been partially solved, namely, the contamination by carcinogenic volatile N-nitrosamines. However, there is still a gap in knowing non-volatile nitroso compounds in terms of both the determination of these compounds and the description of their toxicity. Therefore, a procedure for their detailed non-targeted study is necessary to be developed. Based on these facts, a new method permitting the detection and the classification of nitroso compound groups, such as N-nitroso, C-nitroso, and interfering substances in the nitrosamine specific chemiluminescence detection after previous gas chromatographic separation, was developed. The method is based on signal profiling of chromatographic peaks recorded by a chemiluminescence detector at different pyrolytic temperatures and subsequent multivariate chemometric classification. The resulting classification function by linear discriminant analysis shows good performance with total accuracy of 96.12% after the method validation. The method was successfully applied and demonstrated on a non-targeted beer sample analysis. Nitroso compounds detected by the method were selected for detailed structural analysis by GC-MS/MS. The combination of the presented method with the MS/MS instrumentation provides a really powerful analytical tool for the identification of unknown nitroso compounds in complex samples. This study represents a valuable contribution to the protocols of identification of organic compounds with the nitrogen functional groups - toxicologically and analytically important nitroso compounds" |
| Keywords: | Beer/*analysis Food Contamination/*analysis Gas Chromatography-Mass Spectrometry/methods Luminescent Measurements/*methods Nitrosamines/*analysis Pyrolysis Temperature Chemiluminescence detection Discrimination analysis Gas chromatography Nitrosamines; |
| Notes: | "MedlineVrzal, Tomas Olsovska, Jana eng Netherlands 2019/03/17 Anal Chim Acta. 2019 Jun 20; 1059:136-145. doi: 10.1016/j.aca.2019.01.033. Epub 2019 Jan 25" |
