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Nucleic Acids Res

Title:		"Synthesis, secretion and processing of alpha-factor-interferon fusion proteins in yeast"
Author(s):		Singh A; Lugovoy JM; Kohr WJ; Perry LJ;
Address:
Journal Title:		Nucleic Acids Res
Year:		1984
Volume:		12
Issue:		23
Page Number:		8927 - 8938
DOI:		10.1093/nar/12.23.8927
ISSN/ISBN:		0305-1048 (Print) 1362-4962 (Electronic) 0305-1048 (Linking)
Abstract:		"A gene fusion consisting of 960 base pairs of 5'-flanking region of the yeast MF alpha 1 gene, 257 base pairs coding for alpha-factor prepro sequence, and a modified human IFN-alpha 1 gene was constructed. MAT alpha cells containing the chimeric gene synthesized and secreted active IFN-alpha 1 into the growth medium. The secreted interferon molecules contained the last 4 amino acids of alpha-factor prepro sequence and the amino acids encoded by the DNA modifications introduced at the beginning of IFN-alpha 1 gene. DNA sequences coding for these amino acids were removed by oligonucleotide-directed in vitro mutagenesis. Yeast cells transformed with expression plasmids containing the altered junction synthesized and secreted human IFN-alpha 1 with the natural NH2-terminus"
Keywords:		"Base Sequence *Chimera Chromosome Deletion Coliphages/genetics DNA Restriction Enzymes Escherichia coli/genetics *Genes *Genes, Fungal Humans Interferons/*genetics Mating Factor Mutation Nucleic Acid Conformation Peptides/*genetics Saccharomyces cerevisia;"
Notes:		"MedlineSingh, A Lugovoy, J M Kohr, W J Perry, L J eng England 1984/12/11 Nucleic Acids Res. 1984 Dec 11; 12(23):8927-38. doi: 10.1093/nar/12.23.8927"