| Title: | Simultaneous determination of fifteen multiclass organic pollutants in human saliva and serum by liquid chromatography-tandem ultraviolet/fluorescence detection: A validated method |
| Author(s): | Russo G; Barbato F; Mita DG; Grumetto L; |
| Address: | "Pharm-Analysis & Bio-Pharm Laboratory. Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Naples, Italy. National Laboratory on Endocrine Disruptors, National Institute of Biostructures and Biosystems (INBB), Naples, Italy. Institute of Genetics and Biophysics of CNR, Naples, Italy. Consorzio Interuniversitario INBB, Rome, Italy" |
| ISSN/ISBN: | 1099-0801 (Electronic) 0269-3879 (Linking) |
| Abstract: | "A quick and inexpensive validated method, based on sample treatment by liquid-liquid microextraction followed by liquid chromatography (LC) coupled with ultraviolet tandem fluorescence detection is proposed for the determination of 15 multiclass pollutants both in serum and in saliva, as a simple and easy to draw matrix. The method was set up and validated according to European guidelines. The compounds of interest include some endocrine-disrupting chemicals (i.e. bisphenol A, bisphenol B, bisphenol E, bisphenol F, bisphenol AF, bisphenol A diglycidyl ether, bisphenol M, diethylhexyl phthalate, monoethylhexyl phthalate, triclosan and 4-nonylphenol), as well as other pollutants belonging to the class of volatile organic compounds (2-chlorophenol, 1,2 dichlorobenzene, 1,2,4,5-tetrachlorobenzene). The limits of quantifications ranged from 2.28 x 10(-3) mug mL(-1) (bisphenol A diglycidyl ether) to 6.29 mug mL(-1) (diethylhexyl phthalate), while those of detection ranged from 0.068 x 10(-3) mug mL(-1) (bisphenol A diglycidyl ether) to 1.031 mug mL(-1) (diethylhexyl phthalate). To test method suitability, it was applied to real saliva and serum samples of healthy human volunteers and was found to meet the demands of the laboratories handling simple and relatively inexpensive equipment for screening oriented at rapid and reliable contamination assessment of a population" |
| Keywords: | "Chromatography, Liquid/*methods Endocrine Disruptors/*analysis/blood Environmental Exposure/*analysis Environmental Pollutants/*analysis/blood Humans Limit of Detection Linear Models Reproducibility of Results Saliva/*chemistry Spectrometry, Fluorescence;" |
| Notes: | "MedlineRusso, Giacomo Barbato, Francesco Mita, Damiano Gustavo Grumetto, Lucia eng England 2018/11/09 Biomed Chromatogr. 2019 Mar; 33(3):e4427. doi: 10.1002/bmc.4427. Epub 2018 Dec 3" |
