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J Breath Res

Title:		On-line profiling of volatile compounds produced in vitro by pathogenic oral bacteria
Author(s):		Roslund K; Lehto M; Pussinen P; Groop PH; Halonen L; Metsala M;
Address:		"Department of Chemistry, University of Helsinki, Helsinki, Finland"
Journal Title:		J Breath Res
Year:		2019
Volume:		20191216
Issue:		1
Page Number:		16010 -
DOI:		10.1088/1752-7163/ab5559
ISSN/ISBN:		1752-7163 (Electronic) 1752-7155 (Linking)
Abstract:		"Infections by oral pathogens are one of the most common health problems worldwide. Due to the intimate connection between exhaled breath and the oral cavity, breath analysis could potentially be used to diagnose these infections. However, little is known about the volatile emissions of important oral pathogens that are connected with gingivitis and periodontitis. In this study, we have performed in vitro headspace measurements on four important oral pathogens (P. gingivalis, T. forsythia, P. intermedia and P. nigrescens) using proton transfer reaction time-of-flight mass spectrometry (PTR-TOF-MS). Some of the most abundant compounds produced by the bacteria include hydrogen sulphide, methanethiol, acetone, dimethylsulphide, isoprene, cyclopentanone and indole as tentatively assigned from the mass spectra. Several other abundant mass signals were recorded but the assignment of these is less certain. Some of the bacterial species can be separated from each other by the emitted volatile fingerprints. The results of this study can be used in potential development of a diagnostic breath test for oral infections. In addition, as several of the measured compounds are known to be toxic, the results point to an intriguing possibility of studying the connection between the bacterial virulence and the emitted volatile compounds"
Keywords:		"Automation Bacteria/*metabolism Bacteria, Anaerobic/metabolism Biomarkers/analysis Breath Tests Humans Mass Spectrometry Mouth/*microbiology *Online Systems Reference Standards Volatile Organic Compounds/*analysis;"
Notes:		"MedlineRoslund, Kajsa Lehto, Markku Pussinen, Pirkko Groop, Per-Henrik Halonen, Lauri Metsala, Markus eng Research Support, Non-U.S. Gov't England 2019/11/08 J Breath Res. 2019 Dec 16; 14(1):016010. doi: 10.1088/1752-7163/ab5559"