« Previous AbstractThe Saccharomyces cerevisiae APS1 gene encodes a homolog of the small subunit of the mammalian clathrin AP-1 complex: evidence for functional interaction with clathrin at the Golgi complex    Next AbstractThriving Under Stress: Pseudomonas aeruginosa Outcompetes the Background Polymicrobial Community Under Treatment Conditions in a Novel Chronic Wound Model »

J Breath Res

Title:		Stable isotope profiles reveal active production of VOCs from human-associated microbes
Author(s):		Phan J; Meinardi S; Barletta B; Blake DR; Whiteson K;
Address:		"Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, United States"
Journal Title:		J Breath Res
Year:		2017
Volume:		20170206
Issue:		1
Page Number:		17101 -
DOI:		10.1088/1752-7163/aa5833
ISSN/ISBN:		1752-7163 (Electronic) 1752-7155 (Linking)
Abstract:		"Volatile organic compounds (VOCs) measured from exhaled breath have great promise for the diagnosis of bacterial infections. However, determining human or microbial origin of VOCs detected in breath remains a great challenge. For example, the microbial fermentation product 2,3-butanedione was recently found in the breath of Cystic Fibrosis (CF) patients; parallel culture-independent metagenomic sequencing of the same samples revealed that Streptococcus and Rothia spp. have the genetic capacity to produce 2,3-butanedione. To investigate whether the genetic capacity found in metagenomes translates to bacterial production of a VOC of interest such as 2,3-butanedione, we fed stable isotopes to three bacterial strains isolated from patients: two gram-positive bacteria, Rothia mucilaginosa and Streptococcus salivarius, and a dominant opportunistic gram-negative pathogen, Pseudomonas aeruginosa. Culture headspaces were collected and analyzed using a gas chromatographic system to quantify the abundance of VOCs of interest; mass spectroscopy was used to determine whether the stable isotope label had been incorporated. Our results show that R. mucilaginosa and S. salivarius consumed D-Glucose-(13)C(6) to produce labeled 2,3-butanedione. R. mucilaginosa and S. salivarius also produced labeled acetaldehyde and ethanol when grown with (2)H(2)O. Additionally, we find that P. aeruginosa growth and dimethyl sulfide production are increased when exposed to lactic acid in culture. These results highlight the importance VOCs produced by P. aeruginosa, R. mucilaginosa, and S. salivarius as nutrients and signals in microbial communities, and as potential biomarkers in a CF infection"
Keywords:		Bacteria/*metabolism Cystic Fibrosis/microbiology Humans Isotope Labeling/*methods Metabolome Pseudomonas aeruginosa/metabolism Pyruvates/metabolism Volatile Organic Compounds/*analysis/chemistry;
Notes:		"MedlinePhan, Joann Meinardi, Simone Barletta, Barbara Blake, Donald R Whiteson, Katrine eng England 2017/01/11 J Breath Res. 2017 Feb 6; 11(1):017101. doi: 10.1088/1752-7163/aa5833"