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Genetics

Title:		Self-compatible B mutants in coprinus with altered pheromone-receptor specificities
Author(s):		Olesnicky NS; Brown AJ; Honda Y; Dyos SL; Dowell SJ; Casselton LA;
Address:		"Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom"
Journal Title:		Genetics
Year:		2000
Volume:		156
Issue:		3
Page Number:		1025 - 1033
DOI:		10.1093/genetics/156.3.1025
ISSN/ISBN:		0016-6731 (Print) 0016-6731 (Linking)
Abstract:		"A successful mating in the mushroom Coprinus cinereus brings together a compatible complement of pheromones and G-protein-coupled receptors encoded by multiallelic genes at the B mating-type locus. Rare B gene mutations lead to constitutive activation of B-regulated development without the need for mating. Here we characterize a mutation that arose in the B6 locus and show that it generates a mutant receptor with a single amino acid substitution (R96H) at the intracellular end of transmembrane domain III. Using a heterologous yeast assay and synthetic pheromones we show that the mutation does not make the receptor constitutively active but permits it to respond inappropriately to a normally incompatible pheromone encoded within the same B6 locus. Parallel experiments carried out in Coprinus showed that a F67W substitution in this same pheromone enabled it to activate the normally incompatible wild-type receptor. Together, our experiments show that a single amino acid replacement in either pheromone or receptor can deregulate the specificity of ligand-receptor recognition and confer a self-compatible B phenotype. In addition, we use the yeast assay to demonstrate that different receptors and pheromones found at a single B locus belong to discrete subfamilies within which receptor activation cannot normally occur"
Keywords:		"Amino Acid Sequence Chemoreceptor Cells/*physiology Chromosome Mapping Chromosomes, Fungal/*genetics Coprinus/*genetics Crosses, Genetic Fungal Proteins/genetics/*physiology Genetic Complementation Test Mutagenesis, Site-Directed Pheromones/genetics Prote;"
Notes:		"MedlineOlesnicky, N S Brown, A J Honda, Y Dyos, S L Dowell, S J Casselton, L A eng Research Support, Non-U.S. Gov't 2000/11/07 Genetics. 2000 Nov; 156(3):1025-33. doi: 10.1093/genetics/156.3.1025"