| Title: | Qualitative and quantitative analysis of chemicals emitted from the pheromone gland of individual Heliothis subflexa females |
| Author(s): | Nojima S; Classen A; Groot AT; Schal C; |
| Address: | "Department of Entomology and Plant pathology, North Carolina State University, Raleigh, North Carolina, United States of America. Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands. W.M. Keck Center for Behavioral Biology, North Carolina State University, Raleigh, North Carolina, United States of America" |
| DOI: | 10.1371/journal.pone.0202035 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "The chemicals emitted from the sex pheromone gland of individual Heliothis subflexa females were sampled using a short section of thick-film megabore fused silica capillary column, and the pheromone glands of the same females were extracted after the effluvia collection. Both samples were treated with a silylation reagent, and then subjected to gas chromatography-chemical ionization-mass spectrometry for quantitative and qualitative analysis of all components. The total amount of all 11 components emitted from the glands of calling females was 153 ng/female/hr, which was substantially higher than previously reported. The ratios of the pheromone components in the volatile emissions and pheromone gland extracts were generally similar to previous studies, but with notable differences. The collections of volatiles and gland extractions contained, respectively: Z9-14:Ald (1.57%, 1.35%), 14:Ald (3.78%, 1.51%), Z7 + Z9-16:Ald (9.60%, 3.59%), Z11-16:Ald (76.14%, 18.94%), 16:Ald (2.95%, 2.17%), Z9-16:OH (0.07%, 7.21%), Z11-16:OH (1.11%, 49.04%), Z7-16:OAc (0.48%, 1.73%), Z9-16:OAc (1.32%, 4.02%), and Z11-16:OAc (2.98%, 10.43%). The thick-film megabore column is an efficient approach for sampling the headspace for semiochemicals" |
| Keywords: | Animals Female Gas Chromatography-Mass Spectrometry Moths/*chemistry/*metabolism Reproducibility of Results Scent Glands/*chemistry/*metabolism Sex Attractants/*chemistry/*metabolism Volatile Organic Compounds/chemistry/metabolism; |
| Notes: | "MedlineNojima, Satoshi Classen, Alice Groot, Astrid T Schal, Coby eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2018/08/15 PLoS One. 2018 Aug 14; 13(8):e0202035. doi: 10.1371/journal.pone.0202035. eCollection 2018" |
