| Title: | Targeted mutation of secretogranin-2 disrupts sexual behavior and reproduction in zebrafish |
| Author(s): | Mitchell K; Zhang WS; Lu C; Tao B; Chen L; Hu W; Trudeau VL; |
| Address: | "Department of Biology, University of Ottawa, Ottawa, ON K1N 6N5, Canada. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovation Academy of Seed Design, Chinese Academy of Sciences, 430072 Wuhan, China. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovation Academy of Seed Design, Chinese Academy of Sciences, 430072 Wuhan, China trudeauv@uottawa.ca huwei@ihb.ac.cn. Department of Biology, University of Ottawa, Ottawa, ON K1N 6N5, Canada; trudeauv@uottawa.ca huwei@ihb.ac.cn" |
| ISSN/ISBN: | 1091-6490 (Electronic) 0027-8424 (Print) 0027-8424 (Linking) |
| Abstract: | "The luteinizing hormone surge is essential for fertility as it triggers ovulation in females and sperm release in males. We previously reported that secretoneurin-a, a neuropeptide derived from the processing of secretogranin-2a (Scg2a), stimulates luteinizing hormone release, suggesting a role in reproduction. Here we provide evidence that mutation of the scg2a and scg2b genes using TALENs in zebrafish reduces sexual behavior, ovulation, oviposition, and fertility. Large-scale spawning within-line crossings (n = 82 to 101) were conducted. Wild-type (WT) males paired with WT females successfully spawned in 62% of the breeding trials. Spawning success was reduced to 37% (P = 0.006), 44% (P = 0.0169), and 6% (P < 0.0001) for scg2a(-/-) , scg2b(-/-) , and scg2a(-/-);scg2b(-/-) mutants, respectively. Comprehensive video analysis indicates that scg2a(-/-);scg2b(-/-) mutation reduces all male courtship behaviors. Spawning success was 47% in saline-injected WT controls compared to 11% in saline-injected scg2a(-/-);scg2b(-/-) double mutants. For these mutants, spawning success increased 3-fold following a single intraperitoneal (i.p.) injection of synthetic secretoneurin-a (P = 0.0403) and increased 3.5-fold with injection of human chorionic gonadotropin (hCG). Embryonic survival at 24 h remained on average lower in scg2a(-/-);scg2b(-/-) fish compared to WT injected with secretoneurin-a (P < 0.001). Significant reductions in the expression of gonadotropin-releasing hormone 3 in the hypothalamus, and luteinizing hormone beta and glycoprotein alpha subunits in the pituitary provide evidence for disrupted hypothalamo-pituitary function in scg2a and scg2b mutant fish. Our results indicate that secretogranin-2 is required for optimal reproductive function and support the hypothesis that secretoneurin is a reproductive hormone" |
| Keywords: | "Animals Female *Fertility Gonadotropin-Releasing Hormone/metabolism Hypothalamus/metabolism Luteinizing Hormone/metabolism Male *Mating Preference, Animal *Mutation Neuropeptides/metabolism Oviposition Ovulation Pituitary Gland/metabolism Secretogranin II;" |
| Notes: | "MedlineMitchell, Kimberly Zhang, Wo Su Lu, Chunyu Tao, Binbin Chen, Lu Hu, Wei Trudeau, Vance L eng Research Support, Non-U.S. Gov't 2020/05/30 Proc Natl Acad Sci U S A. 2020 Jun 9; 117(23):12772-12783. doi: 10.1073/pnas.2002004117. Epub 2020 May 28" |
