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J Med Microbiol

Title:		Streptococcus gordonii pheromone s.g.cAM373 may influence the reservoir of antibiotic resistance determinants of Enterococcus faecalis origin in the oral metagenome
Author(s):		Mansfield JM; Herrmann P; Jesionowski AM; Vickerman MM;
Address:		"Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, NY 14214, USA"
Journal Title:		J Med Microbiol
Year:		2017
Volume:		20171012
Issue:		11
Page Number:		1635 - 1639
DOI:		10.1099/jmm.0.000613
ISSN/ISBN:		1473-5644 (Electronic) 0022-2615 (Print) 0022-2615 (Linking)
Abstract:		"Streptococcus gordonii produces a pheromone heptapeptide, s.g.cAM373, which induces a conjugative mating response in Enterococcus faecalis cells carrying the responsive plasmid, pAM373. We investigated the extent of this intergeneric signaling on DNA acquisition by streptococcal species likely to cohabit oral biofilms. E. faecalis/pAM373/pAMS470 cells were incubated with synthetic s.g.cAM373, reverse peptide s.g.cAM373-R, or peptide-free medium and examined for their abilities to transfer plasmid DNA to streptococcal species in the presence of DNase. Preinduction of E. faecalis donors with s.g.cAM373 resulted in transconjugation frequencies in non-pheromone producing strains of Streptococcus mutans, Streptococcus sanguinis, Streptococcus anginosus, and Streptococcus suis that were significantly higher than frequencies when donors were preincubated with s.g.cAM373-R or medium alone. Peptide-mediated communication between commensal streptococci and E. faecalis carrying pheromone-responsive plasmids may facilitate conjugative DNA transfer to bystander species, and influence the reservoir of antibiotic resistance determinants of enterococcal origin in the oral metagenome"
Keywords:		"Anti-Bacterial Agents/*pharmacology Bacterial Proteins/*metabolism/pharmacology Conjugation, Genetic/drug effects DNA, Bacterial Drug Resistance, Bacterial/*physiology Enterococcus faecalis/*drug effects/physiology Gene Expression Regulation, Bacterial Ge;"
Notes:		"MedlineMansfield, Jillian M Herrmann, Paul Jesionowski, Amy M Vickerman, M Margaret eng R01 DE022673/DE/NIDCR NIH HHS/ R21 DE022154/DE/NIDCR NIH HHS/ England 2017/10/13 J Med Microbiol. 2017 Nov; 66(11):1635-1639. doi: 10.1099/jmm.0.000613. Epub 2017 Oct 12"