| Title: | Sex peptide receptor mediates the post-mating switch in Helicoverpa armigera (Lepidoptera: Noctuidae) female reproductive behavior |
| Author(s): | Liu S; Li B; Liu W; Liu Y; Ren B; Wang G; |
| Address: | "Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China. Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: In Drosophila, the sex peptide receptor (SPR) in females to detect male sex peptide (SP) and trigger changes in female behavior including mating rejection and oviposition. In moths, the SPR had been identified and investigated the limited function by using RNA interference (RNAi). However, the detailed function of SPR after a normal mating in moths, especially the role in female sexual receptivity, remains to be elucidated. RESULTS: In this study, we use Helicoverpa armigera, an economically important pest worldwide, as a case study to verify the function of SPR in vivo by CRISPR/Cas9 gene editing system. Analyzing the post-mating behaviors in mutant females, we investigated that HarmSPR could mediate the long-term sexual receptivity (remating, sex pheromone production and calling behavior) switch in female, but not affect the short-term sexual receptivity and the duration time of remating. The oviposition of mated HarmSPR(-/-) females was significantly decreased by ~70% comparing to wild-type (WT) mated females and there were more eggs staying in the ovary of mutant females. SPR also showed effect on female longevity, which is that the mated HarmSPR(-/-) females showed longer longevity than mated WT females. CONCLUSION: The results detailedly demonstrated the functional role of SPR in female post-mating behaviors and we could propose a pest control strategy in which developing an antagonist of SPR to reduce the oviposition of female. (c) 2021 Society of Chemical Industry" |
| Keywords: | "Animals Female Insect Proteins/genetics Male *Moths/genetics *Receptors, Peptide/genetics Reproduction *Sex Attractants/physiology *Sexual Behavior, Animal CRISPR/Cas9 Helicoverpa armigera Spr pest control post-mating behavior;" |
| Notes: | "MedlineLiu, Shuai Li, Bin Liu, Wei Liu, Yang Ren, Bingzhong Wang, Guirong eng 31725023/National Natural Science Foundation of China/ 31861133019/National Natural Science Foundation of China/ 31911530234/National Natural Science Foundation of China/ KQTD20180411143628272/Shenzhen Science and Technology Program/ England 2021/04/03 Pest Manag Sci. 2021 Jul; 77(7):3427-3435. doi: 10.1002/ps.6391. Epub 2021 Apr 19" |
