| Title: | Quantification of volatile organic compounds by secondary electrospray ionization-high resolution mass spectrometry |
| Author(s): | Liu C; Zeng J; Sinues P; Fang M; Zhou Z; Li X; |
| Address: | "Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou, 510632, China. Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou, 510632, China; Department of Biomedical Engineering, Basel University, Allschwil, 4123, Switzerland; University of Basel Children's Hospital (UKBB), Basel, 4056, Switzerland. Department of Biomedical Engineering, Basel University, Allschwil, 4123, Switzerland; University of Basel Children's Hospital (UKBB), Basel, 4056, Switzerland. School of Civil and Environmental Engineering, Nanyang Technological University, Singapore, 639798, Singapore. Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou, 510632, China; Guangzhou Hexin Instrument Co., LTD., Guangzhou, 510530, China; Guangdong-Hongkong-Macau Joint Laboratory of Collaborative Innovation for Environmental Quality, Guangzhou, 510632, China. Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou, 510632, China; Guangzhou Hexin Instrument Co., LTD., Guangzhou, 510530, China; Guangdong-Hongkong-Macau Joint Laboratory of Collaborative Innovation for Environmental Quality, Guangzhou, 510632, China. Electronic address: tamylee@jnu.edu.cn" |
| DOI: | 10.1016/j.aca.2021.338876 |
| ISSN/ISBN: | 1873-4324 (Electronic) 0003-2670 (Linking) |
| Abstract: | "Secondary electrospray ionization coupled with high resolution mass spectrometry (SESI-HRMS) is a direct mass spectrometry technique, which can identify trace volatile organic compounds (VOCs) in real time without sample pretreatment and chromatographic separation. SESI-HRMS has been successfully applied in multiple applications, including breath analysis, animals and plants VOCs emissions, analysis of headspace of cell cultures and indoor and outdoor air. The range of areas where the technique can potentially have a substantial impact is very broad. However, one critical aspect that requires further development to consolidate the technique is absolute quantification. Therefore, in this study we aim to develop a quantitative method for eight representative VOCs, including ketones (acetone, 2-butanone and 2-pentanone), alkenes (isoprene and alpha-terpinene) and aromatics (toluene, styrene and mesitylene). The mass spectrometric platform includes a commercial SESI source hyphenated with a Q-Exactive hybrid quadrupole Orbitrap high resolution mass spectrometer. Within the concentration range of 0-100 ppbv studied, the optimal coefficient of determination for linear regression (R(2) = 0.993-0.999) between signal intensity and concentration is obtained in the range of 0-10 ppbv for all eight VOCs. The detection limits range between 3 (2-Pentanone) and 15 (Acetone) pptv. The intra-day (n = 10) and inter-day (n = 30) coefficients of variation (CV) are |
| Keywords: | "Acetone Breath Tests Spectrometry, Mass, Electrospray Ionization *Volatile Organic Compounds High resolution mass spectrometry Quantitative analysis Secondary electrospray ionization Volatile organic compounds;" |
| Notes: | "MedlineLiu, Chao Zeng, Jiafa Sinues, Pablo Fang, Mingliang Zhou, Zhen Li, Xue eng Netherlands 2021/09/21 Anal Chim Acta. 2021 Oct 2; 1180:338876. doi: 10.1016/j.aca.2021.338876. Epub 2021 Jul 26" |
