| Title: | Targeted and Intracellular Antibacterial Activity against S. agalactiae of the Chimeric Peptides Based on Pheromone and Cell-Penetrating Peptides |
| Author(s): | Li J; Shang L; Lan J; Chou S; Feng X; Shi B; Wang J; Lyu Y; Shan A; |
| Address: | "Institute of Animal Nutrition, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin 150030, P. R. China" |
| Journal Title: | ACS Appl Mater Interfaces |
| ISSN/ISBN: | 1944-8252 (Electronic) 1944-8244 (Linking) |
| Abstract: | "The significance of the complex bacterial ecosystem in the human body and the impediment of the mammalian membrane against many antibiotics together emphasize the necessity to develop antimicrobial agents with precise antimicrobial and cell-penetrating activities. A simple and feasible method for generating dual-function antimicrobial peptides inspired by highly hydrophobic peptide pheromone and cationic cell-penetrating peptides is presented. Furthermore, the extension of the peptide candidate library is achieved by modifying the charged domain. The bacteria-selective peptides L1, L2, L10, and L11 kill Streptococcus agalactiae by disrupting the membrane structure, and the targeted mechanism is suggested where the peptides offset the entrapment of S. agalactiae rather than of other bacteria. Moreover, L2 and L10 possess intracellular antibacterial activity and carrier property, which is mainly dependent on endocytosis. Given their suitable biocompatibility, high tolerance, no drug resistance, and effective antimicrobial capacity in a mouse mastitis model, L2 and L10 can be powerful weapons against S. agalactiae pathogen infection" |
| Keywords: | "Animals Anti-Bacterial Agents/chemistry/*pharmacology Antimicrobial Cationic Peptides/chemistry/*pharmacology Cell Survival/drug effects Cell-Penetrating Peptides/chemistry/*pharmacology Dose-Response Relationship, Drug Erythrocytes/drug effects HEK293 Ce;" |
| Notes: | "MedlineLi, Jiawei Shang, Lu Lan, Jing Chou, Shuli Feng, Xingjun Shi, Baoming Wang, Jiajun Lyu, Yinfeng Shan, Anshan eng 2020/09/15 ACS Appl Mater Interfaces. 2020 Oct 7; 12(40):44459-44474. doi: 10.1021/acsami.0c12226. Epub 2020 Sep 25" |
