| Title: | A yeast operator overlaps an upstream activation site |
| Author(s): | Kronstad JW; Holly JA; MacKay VL; |
| DOI: | 10.1016/0092-8674(87)90491-0 |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "The product of the BAR1 gene of Saccharomyces cerevisiae is synthesized only in the a cell type and inactivates alpha-factor, the mating pheromone made by alpha cells. The MAT alpha 2 protein represses the transcription of a-cell-specific genes, including BAR1, in alpha and a/alpha diploid cells. Transcription of BAR1 in a cells in stimulated upon exposure to alpha-factor. Deletion analysis of the 5' noncoding region of the BAR1 gene revealed that the major upstream activation site (UAS) overlaps the 31 bp operator sequence required for MAT alpha 2 repression. This result has implications for the negative control of transcription in yeast. The deletion analysis also indicated that the sequence TGAAACA mediates alpha-factor stimulation" |
| Keywords: | "Base Sequence *Gene Expression Regulation *Genes, Fungal Mutation *Operator Regions, Genetic Pheromones/genetics Repressor Proteins/genetics Saccharomyces cerevisiae/*genetics Transcription, Genetic;" |
| Notes: | "MedlineKronstad, J W Holly, J A MacKay, V L eng Research Support, U.S. Gov't, Non-P.H.S. 1987/07/31 Cell. 1987 Jul 31; 50(3):369-77. doi: 10.1016/0092-8674(87)90491-0" |
