| Title: | "Cloning, functional characterization and expression analysis of LoTPS5 from Lilium 'Siberia'" |
| Author(s): | Abbas F; Ke Y; Zhou Y; Waseem M; Yu Y; Ashraf U; Li X; Wang C; Yue Y; Yu R; Fan Y; |
| Address: | "The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Horticulture, South China Agricultural University, Guangzhou 51642, China. Department of Botany, Division of Science and Technology, University of Education, Lahore 54770, Punjab, Pakistan. College of Life Sciences, South China Agricultural University, Guangzhou 510642, China. Electronic address: rcyu@scau.edu.cn. The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou 510642, China. Electronic address: fanyanping@scau.edu.cn" |
| DOI: | 10.1016/j.gene.2020.144921 |
| ISSN/ISBN: | 1879-0038 (Electronic) 0378-1119 (Linking) |
| Abstract: | "Lilium 'Siberia' is a perennial herbaceous plant that is commercially significant because of its snowy white floral color and appealing scent which is mainly due to the presence of monoterpenes and benzoids compounds in floral volatile profile. In the current study, LoTPS5 was cloned and functionally characterized. Results revealed that LoTPS5 specifically generates squalene from FPP, whereas no product was produced when it was incubated with GPP or GGPP. The subcellular localization experiment showed that LoTPS5 was located in plastids. Furthermore, LoTPS5 showed its high expression in the leaf followed by petals and sepals of the flower. Moreover, the expression of LoTPS5 gradually increased from the bud stage and peak at the full-bloom stage. Besides, LoTPS5 showed a diurnal circadian rhythmic pattern with a peak in the afternoon (16:00) followed by deep night (24:00) and morning (8:00), respectively. LoTPS5 is highly responsive to mechanical wounding by rapidly elevating its mRNA transcript level. The current study will provide significant information for future studies of terpenoid and squalene biosynthesis in Lilium 'Siberia'" |
| Keywords: | "Amino Acid Sequence Biosynthetic Pathways Cloning, Molecular Farnesyl-Diphosphate Farnesyltransferase/analysis/chemistry/*genetics Gene Expression Gene Expression Regulation, Plant Lilium/chemistry/*enzymology/*genetics/metabolism Odorants/analysis Phylog;" |
| Notes: | "MedlineAbbas, Farhat Ke, Yanguo Zhou, Yiwei Waseem, Muhammad Yu, Yunyi Ashraf, Umair Li, Xinyue Wang, Chutian Yue, Yuechong Yu, Rangcai Fan, Yanping eng Netherlands 2020/07/01 Gene. 2020 Sep 25; 756:144921. doi: 10.1016/j.gene.2020.144921. Epub 2020 Jun 25" |
