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Behav Brain Res

Title:		Sex differences in olfactory-induced neural activation of the amygdala
Author(s):		Kikusui T; Kajita M; Otsuka N; Hattori T; Kumazawa K; Watarai A; Nagasawa M; Inutsuka A; Yamanaka A; Matsuo N; Covington HE; Mogi K;
Address:		"Companion Animal Research, School of Veterinary Medicine, Azabu University, Sagamihara 252-5201, Japan. Companion Animal Research, School of Veterinary Medicine, Azabu University, Sagamihara 252-5201, Japan; Division of Brain and Neurophysiology, Department of Physiology, Jichi Medical University, Yakushiji 3311-1, Shimotsuke, Tochigi 329-0498, Japan. Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan; Division of Brain and Neurophysiology, Department of Physiology, Jichi Medical University, Yakushiji 3311-1, Shimotsuke, Tochigi 329-0498, Japan. Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan. Department of Molecular and Behavioral Neuroscience, Graduate School of Medicine, Osaka University, Suita 565-0871, Japan. Laboratory of Psychopharmacology, Department of Psychology, Tufts University,530 Boston Avenue, Medford, MA 02155, USA. Companion Animal Research, School of Veterinary Medicine, Azabu University, Sagamihara 252-5201, Japan. Electronic address: mogik@azabu-u.ac.jp"
Journal Title:		Behav Brain Res
Year:		2018
Volume:		20171202
Issue:
Page Number:		96 - 104
DOI:		10.1016/j.bbr.2017.11.034
ISSN/ISBN:		1872-7549 (Electronic) 0166-4328 (Linking)
Abstract:		"Olfactory signals, including the scent of urine, are thought to be processed by specific brain regions, such as the medial amygdala (Me), and regulate sexual behavior in a sex-dependent manner. We aimed to reveal the sex-specific neural circuit from the accessory olfactory bulb (AOB) to Me by using a transgenic mouse. We quantified the long-lasting green fluorescent protein (GFP) expression profile, which was controlled by the c-fos promotor in a sex-dependent manner by the scent of urine. Female urine predominantly activated neurons of the posterodorsal medial amygdala (MePD) in male mice and the posteroventral medial amygdala (MePV) in female mice. Male urine, in contrast, generated the opposite pattern of activation in the Me. Secondary, the selective artificial activation of these circuits was used to examine their specific behavioral function, by using a dual Cre-loxP viral infection. AAV-hSyn-FLEX-hM3Dq-EGFP-the designer receptor exclusively activated by a designer drug-was infused into the AOB after infection with trans-synaptic AAV(DJ)-CMV-mCherry-2A-Cre-TTC into either the MePD or the MePV. Double virus-transfected mice were injected with hM(3)Dq activator and their sexual behavior was monitored. However, selective activation of sex-dependent circuits, i.e., the AOB-MePD or AOB-MePV, did not significantly alter mounting or attack behavior in male mice. There were clear sex differences in the pheromone conveying circuits in the AOB-Me of mice. The sex-dependent functional activation of the Me, however, no effect on behavior. This suggests that a diverse number of nuclei and brain areas are likely to function in concert to successfully facilitate sexual and aggressive behaviors"
Keywords:		"Amygdala/cytology/*physiology Animals Early Growth Response Protein 1/metabolism Female Green Fluorescent Proteins/genetics/metabolism Male Mice, Inbred C57BL Mice, Transgenic Neurons/cytology/drug effects/*physiology Olfactory Bulb/cytology/drug effects/;Neuroscience;"
Notes:		"MedlineKikusui, Takefumi Kajita, Mayu Otsuka, Natsumi Hattori, Tatsuya Kumazawa, Kanako Watarai, Akiyuki Nagasawa, Miho Inutsuka, Ayumu Yamanaka, Akihiro Matsuo, Naoki Covington, Herbert E 3rd Mogi, Kazutaka eng Research Support, Non-U.S. Gov't Netherlands 2017/12/06 Behav Brain Res. 2018 Jul 2; 346:96-104. doi: 10.1016/j.bbr.2017.11.034. Epub 2017 Dec 2"