| Title: | Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project |
| Author(s): | Ahmed WM; Brinkman P; Weda H; Knobel HH; Xu Y; Nijsen TM; Goodacre R; Rattray N; Vink TJ; Santonico M; Pennazza G; Montuschi P; Sterk PJ; Fowler SJ; |
| Address: | "School of Biological Sciences, University of Manchester, United Kingdom" |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies" |
| Keywords: | Asthma/*diagnosis Biomarkers/analysis Breath Tests/*methods Cohort Studies Databases as Topic Humans Multivariate Analysis Reference Standards Volatile Organic Compounds/analysis; |
| Notes: | "MedlineAhmed, Waqar M Brinkman, Paul Weda, Hans Knobel, Hugo H Xu, Yun Nijsen, Tamara M Goodacre, Royston Rattray, Nicholas Vink, Teunis J Santonico, Marco Pennazza, Giorgio Montuschi, Paolo Sterk, Peter J Fowler, Stephen J eng Research Support, Non-U.S. Gov't England 2018/10/03 J Breath Res. 2018 Oct 30; 13(1):016001. doi: 10.1088/1752-7163/aae557" |
