| Title: | Pith-specific lignification in Nicotiana attenuata as a defense against a stem-boring herbivore |
| Author(s): | Joo Y; Kim H; Kang M; Lee G; Choung S; Kaur H; Oh S; Choi JW; Ralph J; Baldwin IT; Kim SG; |
| Address: | "Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, Jena, 07745, Germany. Department of Biological Sciences, Korea Advanced Institute for Science and Technology, Daejeon, 34141, Korea. Department of Biology, Chungbuk National University, Cheongju, 28644, Korea. Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin-Madison, 1552 University Ave., Madison, WI, 53726, USA. Department of Biochemistry, University of Wisconsin-Madison, 1552 University Ave., Madison, WI, 53726, USA. Graduate School of International Agricultural Technology, Seoul National University, Pyoeng-Chang, 25354, Korea" |
| ISSN/ISBN: | 1469-8137 (Electronic) 0028-646X (Linking) |
| Abstract: | "Plants have developed tissue-specific defense strategies in response to various herbivores with different feeding habits. Although defense responses to leaf-chewing insects have been well studied, little is known about stem-specific responses, particularly in the pith, to stem-boring herbivores. To understand the stem-specific defense, we first conducted a comparative transcriptomic analysis of the wild tobacco Nicotiana attenuata before and after attack by the leaf-chewing herbivore Manduca sexta and the stem borer Trichobaris mucorea. When the stem-boring herbivore attacked, lignin-associated genes were upregulated specifically in the inner parenchymal cells of the stem, the pith; lignin also accumulated highly in the attacked pith. Silencing the lignin biosynthetic gene cinnamyl alcohol dehydrogenase enhanced the performance of the stem-boring herbivore but had no effect on the growth of the leaf-chewing herbivore. Two-dimensional nuclear magnetic resonance results revealed that lignified pith contains feruloyltyramine as an unusual lignin component in the cell wall, as a response against stem-boring herbivore attack. Pith-specific lignification induced by the stem-boring herbivore was modulated by both jasmonate and ethylene signaling. These results suggest that lignin provides a stem-specific inducible barrier, protecting plants against stem-boring insects" |
| Keywords: | "Animals Gene Expression Regulation, Plant Herbivory *Manduca Tobacco/genetics *Weevils Trichobaris mucorea cinnamyl alcohol dehydrogenase (CAD) ethylene feruloyltyramine herbivore-induced lignification jasmonate pith plant defense;" |
| Notes: | "MedlineJoo, Youngsung Kim, Hoon Kang, Moonyoung Lee, Gisuk Choung, Sungjun Kaur, Harleen Oh, Shinyoung Choi, Jun Weon Ralph, John Baldwin, Ian T Kim, Sang-Gyu eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2021/06/26 New Phytol. 2021 Oct; 232(1):332-344. doi: 10.1111/nph.17583. Epub 2021 Jul 22" |
