| Title: | Screening of volatile organic compounds (VOCs) from liquid fungal cultures using ambient mass spectrometry |
| Author(s): | Heffernan D; Pilz M; Klein M; Haack M; Race AM; Bruck T; Qoura F; Strittmatter N; |
| Address: | "Department of Biosciences, TUM School of Natural Sciences, Technical University of Munich (TUM), Garching, Germany. Department of Chemistry, TUM School of Natural Sciences, Technical University of Munich (TUM), Garching, Germany. Institute of Medical Bioinformatics and Biostatistics, University of Marburg, Marburg, Germany. Department of Biosciences, TUM School of Natural Sciences, Technical University of Munich (TUM), Garching, Germany. nicole.strittmatter@tum.de" |
| DOI: | 10.1007/s00216-023-04769-6 |
| ISSN/ISBN: | 1618-2650 (Electronic) 1618-2642 (Print) 1618-2642 (Linking) |
| Abstract: | "The potential of fungi for use as biotechnological factories in the production of a range of valuable metabolites, such as enzymes, terpenes, and volatile aroma compounds, is high. Unlike other microorganisms, fungi mostly secrete secondary metabolites into the culture medium, allowing for easy extraction and analysis. To date, the most commonly used technique in the analysis of volatile organic compounds (VOCs) is gas chromatography, which is time and labour consuming. We propose an alternative ambient screening method that provides rapid chemical information for characterising the VOCs of filamentous fungi in liquid culture using a commercially available ambient dielectric barrier discharge ionisation (DBDI) source connected to a quadrupole-Orbitrap mass spectrometer. The effects of method parameters on measured peak intensities of a series of 8 selected aroma standards were optimised with the best conditions being selected for sample analysis. The developed method was then deployed to the screening of VOCs from samples of 13 fungal strains in three different types of complex growth media showing clear differences in VOC profiles across the different media, enabling determination of best culturing conditions for each compound-strain combination. Our findings underline the applicability of ambient DBDI for the direct detection and comparison of aroma compounds produced by filamentous fungi in liquid culture" |
| Keywords: | *Volatile Organic Compounds/analysis Gas Chromatography-Mass Spectrometry/methods Mass Spectrometry Culture Media/analysis Fungi Ambient mass spectrometry Dielectric barrier discharge ionisation (DBDI) Headspace analysis Volatile organic compounds (VOCs); |
| Notes: | "MedlineHeffernan, Daniel Pilz, Melania Klein, Marco Haack, Martina Race, Alan M Bruck, Thomas Qoura, Farah Strittmatter, Nicole eng Germany 2023/06/30 Anal Bioanal Chem. 2023 Jul; 415(18):4615-4627. doi: 10.1007/s00216-023-04769-6. Epub 2023 Jun 30" |
