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Food Chem

Title:		"One-step column chromatographic extraction with gradient elution followed by automatic separation of volatiles, flavonoids and polysaccharides from Citrus grandis"
Author(s):		Han HB; Li H; Hao RL; Chen YF; Ni H; Li HH;
Address:		"College of Chemistry and Life Sciences, Guangdong University of Petrochemical Technology, 139 Guandu Road 2, Maoming City, Guangdong 525000, China; Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China"
Journal Title:		Food Chem
Year:		2014
Volume:		20130903
Issue:
Page Number:		542 - 548
DOI:		10.1016/j.foodchem.2013.08.092
ISSN/ISBN:		1873-7072 (Electronic) 0308-8146 (Linking)
Abstract:		"Citrus grandis Tomentosa is widely used in traditional Chinese medicine and health foods. Its functional components include volatiles, flavonoids and polysaccharides which cannot be effectively extracted through traditional methods. A column chromatographic extraction with gradient elution was developed for one-step extraction of all bioactive substances from C. grandis. Dried material was loaded into a column with petroleum ether: ethanol (8:2, PE) and sequentially eluted with 2-fold PE, 3-fold ethanol: water (6:4) and 8-fold water. The elutes was separated into an ether fraction containing volatiles and an ethanol-water fraction containing flavonoids and polysaccharides. The later was separated into flavonoids and polysaccharides by 80% ethanol precipitation of polysaccharides. Through this procedure, volatiles, flavonoids and polysaccharides in C. grandis were simultaneously extracted at 98% extraction rates and simply separated at higher than 95% recovery rates. The method provides a simple and high-efficient extraction and separation of wide range bioactive substances"
Keywords:		"Chromatography/*methods Chromatography, High Pressure Liquid Citrus/*chemistry Cyclohexenes/analysis Flavonoids/*analysis Food Analysis Limonene Plant Extracts/analysis Polysaccharides/*analysis Terpenes/analysis Volatile Organic Compounds/*analysis Citru;"
Notes:		"MedlineHan, Han-Bing Li, Hui Hao, Rui-Lin Chen, Ya-Fei Ni, He Li, Hai-Hang eng Research Support, Non-U.S. Gov't England 2013/10/17 Food Chem. 2014 Feb 15; 145:542-8. doi: 10.1016/j.foodchem.2013.08.092. Epub 2013 Sep 3"