| Title: | Sniffing fungi - phenotyping of volatile chemical diversity in Trichoderma species |
| Author(s): | Guo Y; Jud W; Ghirardo A; Antritter F; Benz JP; Schnitzler JP; Rosenkranz M; |
| Address: | "Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum Munchen, D-85764, Neuherberg, Germany. Holzforschung Munchen, TUM School of Life Sciences Weihenstephan, Technical University of Munich, D-85354, Freising, Germany" |
| ISSN/ISBN: | 1469-8137 (Electronic) 0028-646X (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) play vital roles in the interaction of fungi with plants and other organisms. A systematic study of the global fungal VOC profiles is still lacking, though it is a prerequisite for elucidating the mechanisms of VOC-mediated interactions. Here we present a versatile system enabling a high-throughput screening of fungal VOCs under controlled temperature. In a proof-of-principle experiment, we characterized the volatile metabolic fingerprints of four Trichoderma spp. over a 48 h growth period. The developed platform allows automated and fast detection of VOCs from up to 14 simultaneously growing fungal cultures in real time. The comprehensive analysis of fungal odors is achieved by employing proton transfer reaction-time of flight-MS and GC-MS. The data-mining strategy based on multivariate data analysis and machine learning allows the volatile metabolic fingerprints to be uncovered. Our data revealed dynamic, development-dependent and extremely species-specific VOC profiles from the biocontrol genus Trichoderma. The two mass spectrometric approaches were highly complementary to each other, together revealing a novel, dynamic view to the fungal VOC release. This analytical system could be used for VOC-based chemotyping of diverse small organisms, or more generally, for any in vivo and in vitro real-time headspace analysis" |
| Keywords: | Gas Chromatography-Mass Spectrometry Mass Spectrometry Odorants/analysis *Trichoderma *Volatile Organic Compounds/analysis Trichoderma Gc-ms Proton transfer reaction-time of flight-MS automated cuvettes chemical diversity data mining fungi volatile organi; |
| Notes: | "MedlineGuo, Yuan Jud, Werner Ghirardo, Andrea Antritter, Felix Benz, J Philipp Schnitzler, Jorg-Peter Rosenkranz, Maaria eng Research Support, Non-U.S. Gov't England 2020/03/11 New Phytol. 2020 Jul; 227(1):244-259. doi: 10.1111/nph.16530. Epub 2020 Apr 23" |
