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J Breath Res

Title:		Targeted breath analysis: exogenous volatile organic compounds (EVOC) as metabolic pathway-specific probes
Author(s):		Gaude E; Nakhleh MK; Patassini S; Boschmans J; Allsworth M; Boyle B; van der Schee MP;
Address:		"Owlstone Medical, 183 Cambridge Science Park, Milton Road, Cambridge CB4 0GJ, United Kingdom"
Journal Title:		J Breath Res
Year:		2019
Volume:		20190517
Issue:		3
Page Number:		32001 -
DOI:		10.1088/1752-7163/ab1789
ISSN/ISBN:		1752-7163 (Electronic) 1752-7155 (Linking)
Abstract:		"Breath research has almost invariably focussed on the identification of endogenous volatile organic compounds (VOCs) as disease biomarkers. After five decades, a very limited number of breath tests measuring endogenous VOCs is applied to the clinic. In this perspective article, we explore some of the factors that may have contributed to the current lack of clinical applications of breath endogenous VOCs. We discuss potential pitfalls of experimental design, analytical challenges, as well as considerations regarding the biochemical pathways that may impinge on the application of endogenous VOCs as specific disease biomarkers. We point towards several lines of evidence showing that breath analysis based on administration of exogenous compounds has been a more successful strategy, with several tests currently applied to the clinic, compared to measurement of endogenous VOCs. Finally, we propose a novel approach, based on the use of exogenous VOC (EVOC) probes as potential strategy to measure the activity of metabolic enzymes in vivo, as well as the function of organs, through breath analysis. We present longitudinal data showing the potential of EVOC probe strategies in breath analysis. We also gathered important data showing that administration of EVOC probes induces significant changes compared to previous exposures to the same compounds. EVOC strategies could herald a new wave of substrate-based breath tests, potentially bridging the gap between research tools and clinical applications"
Keywords:		Breath Tests/*methods Humans Metabolic Networks and Pathways/*physiology Volatile Organic Compounds/analysis/*chemistry;
Notes:		"MedlineGaude, Edoardo Nakhleh, Morad K Patassini, Stefano Boschmans, Jasper Allsworth, Max Boyle, Billy van der Schee, Marc P eng England 2019/04/10 J Breath Res. 2019 May 17; 13(3):032001. doi: 10.1088/1752-7163/ab1789"