| Title: | "Scooter, a new active transposon in Schizophyllum commune, has disrupted two genes regulating signal transduction" |
| Address: | "Microbiology and Molecular Genetics Department, University of Vermont, Burlington, Vermont 05405, USA. tfowler@zoo.uvm.edu" |
| DOI: | 10.1093/genetics/156.4.1585 |
| ISSN/ISBN: | 0016-6731 (Print) 0016-6731 (Linking) |
| Abstract: | "Two copies of scooter, a DNA-mediated transposon in the basidiomycetous fungus Schizophyllum commune, were characterized. Scooter is the first transposon isolated from S. commune. Scooter creates 8-bp target site duplications, comparable to members of the hAT superfamily, and has 32-bp terminal inverted repeats. Both copies of scooter are nonautonomous elements capable of movement. Southern blot hybridizations show that scooter-related sequences are present in all S. commune strains tested. Scooter-1 was identified initially as an insertion in the Bbeta2 pheromone receptor gene, bbr2, leading to a partial defect in mating. Scooter-2 spontaneously disrupted a gene to produce the frequently occurring morphological mutant phenotype known as thin. The scooter-2 insert permitted cloning of the disrupted gene, thn1, which encodes a putative regulator of G protein signaling (RGS) protein. Spontaneous insertion of scooter into genes with identifiable mutant phenotypes constitutes the first evidence of active transposition of a DNA-mediated transposon in a basidiomycete" |
| Keywords: | "Amino Acid Sequence Bacterial Proteins/chemistry/*genetics Base Sequence Blotting, Southern Cloning, Molecular DNA Transposable Elements/*genetics DNA, Fungal/genetics Fungal Proteins/*genetics/physiology GTP-Binding Proteins/metabolism Genes, Fungal Mole;" |
| Notes: | "MedlineFowler, T J Mitton, M F eng Comparative Study Research Support, U.S. Gov't, Non-P.H.S. 2000/12/05 Genetics. 2000 Dec; 156(4):1585-94. doi: 10.1093/genetics/156.4.1585" |
