| Title: | Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast |
| Author(s): | Emmerstorfer-Augustin A; Pichler H; |
| Address: | "ACIB - Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010, Graz, Austria. anita.emmerstorfer@acib.at. ACIB - Austrian Centre of Industrial Biotechnology, Petersgasse 14, 8010, Graz, Austria. Institute of Molecular Biotechnology, NAWI Graz, Graz University of Technology, Petersgasse 14/2, 8010, Graz, Austria" |
| DOI: | 10.1007/978-1-4939-3393-8_8 |
| ISSN/ISBN: | 1940-6029 (Electronic) 1064-3745 (Linking) |
| Abstract: | "Plant terpenoids are high-value compounds broadly applied as food additives or fragrances in perfumes and cosmetics. Their biotechnological production in yeast offers an attractive alternative to extraction from plants. Here, we provide two optimized protocols for the production of the plant terpenoid trans-nootkatol with recombinant S. cerevisiae by either (I) converting externally added (+)-valencene with resting cells or (II) cultivating engineered self-sufficient production strains. By synthesis of the hydrophobic compounds in self-sufficient production cells, phase transfer issues can be avoided and the highly volatile products can be enriched in and easily purified from n-dodecane, which is added to the cell broth as second phase" |
| Keywords: | Biotransformation Metabolic Engineering Plants/*chemistry Saccharomyces cerevisiae/genetics/*metabolism Terpenes/*metabolism Volatile Organic Compounds/metabolism (+)-Valencene synthase Cytochrome P450 enzymes Plant terpenoids S.cerevisiae Yeast tHMG1; |
| Notes: | "MedlineEmmerstorfer-Augustin, Anita Pichler, Harald eng Research Support, Non-U.S. Gov't 2016/02/05 Methods Mol Biol. 2016; 1405:79-89. doi: 10.1007/978-1-4939-3393-8_8" |
