| Title: | A sulfur-containing volatile emitted by potato-associated bacteria confers protection against late blight through direct anti-oomycete activity |
| Author(s): | Chinchilla D; Bruisson S; Meyer S; Zuhlke D; Hirschfeld C; Joller C; L'Haridon F; Mene-Saffrane L; Riedel K; Weisskopf L; |
| Address: | "Department of Biology, University of Fribourg, Chemin du Musee 10, CH-1700, Fribourg, Switzerland. Department of Microbial Physiology and Molecular Biology, University of Greifswald, Felix-Hausdorff-Strasse 8, D-17489, Greifswald, Germany. Department of Microbial Proteomics, University of Greifswald, Felix-Hausdorff-Strasse 8, D-17489, Greifswald, Germany. Department of Biology, University of Fribourg, Chemin du Musee 10, CH-1700, Fribourg, Switzerland. laure.weisskopf@unifr.ch" |
| DOI: | 10.1038/s41598-019-55218-3 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Plant diseases are a major cause for yield losses and new strategies to control them without harming the environment are urgently needed. Plant-associated bacteria contribute to their host's health in diverse ways, among which the emission of disease-inhibiting volatile organic compounds (VOCs). We have previously reported that VOCs emitted by potato-associated bacteria caused strong in vitro growth inhibition of the late blight causing agent Phytophthora infestans. This work focuses on sulfur-containing VOCs (sVOCs) and demonstrates the high in planta protective potential of S-methyl methane thiosulfonate (MMTS), which fully prevented late blight disease in potato leaves and plantlets without phytotoxic effects, in contrast to other sVOCs. Short exposure times were sufficient to protect plants against infection. We further showed that MMTS's protective activity was not mediated by the plant immune system but lied in its anti-oomycete activity. Using quantitative proteomics, we determined that different sVOCs caused specific proteome changes in P. infestans, indicating perturbations in sulfur metabolism, protein translation and redox balance. This work brings new perspectives for plant protection against the devastating Irish Famine pathogen, while opening new research avenues on the role of sVOCs in the interaction between plants and their microbiome" |
| Keywords: | Phytophthora infestans/*growth & development Plant Diseases/*microbiology/parasitology Plant Leaves/metabolism Plant Proteins/metabolism Solanum tuberosum/metabolism/*microbiology/parasitology Sulfur/*metabolism Volatile Organic Compounds/*metabolism; |
| Notes: | "MedlineChinchilla, Delphine Bruisson, Sebastien Meyer, Silvan Zuhlke, Daniela Hirschfeld, Claudia Joller, Charlotte L'Haridon, Floriane Mene-Saffrane, Laurent Riedel, Katharina Weisskopf, Laure eng Research Support, Non-U.S. Gov't England 2020/01/01 Sci Rep. 2019 Dec 30; 9(1):18778. doi: 10.1038/s41598-019-55218-3" |
