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Mol Cell Biochem

Title:		"Putative volatile biomarkers of bovine tuberculosis infection in breath, skin and feces of cattle"
Author(s):		Brebu M; Simion VE; Andronie V; Jaimes-Mogollon AL; Beleno-Saenz KJ; Ionescu F; Welearegay TG; Suschinel R; de Lema JB; Ionescu R;
Address:		"'Petru Poni' Institute of Macromolecular Chemistry, Aleea Grigore Ghica Voda 41A, 700487, Iasi, Romania. Faculty of Veterinary Medicine, Spiru Haret University, Bd. Basarabia 256, 030171, Bucharest, Romania. GISM Group, Faculty of Engineering and Architecture, University of Pamplona, Ciudad Universitaria, Via Bucaramanga Km 1, 543050, Pamplona, Colombia. Department of Mechatronics Engineering, Universidad Autonoma del Caribe, Calle 90 #46-112, 080020, Barranquilla, Colombia. The Angstrom Laboratory, Department of Materials Science and Engineering, Uppsala University, P.O. Box 35, 75103, Uppsala, Sweden. Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Kreutzwaldi 62, 51006, Tartu, Estonia. D Asociation, Paseo de la Montana 14, 08402, Granollers, Barcelona, Spain. D Asociation, Paseo de la Montana 14, 08402, Granollers, Barcelona, Spain. SeptimiuRadu.Ionescu@emu.ee"
Journal Title:		Mol Cell Biochem
Year:		2023
Volume:		20230225
Issue:
Page Number:		-
DOI:		10.1007/s11010-023-04676-5
ISSN/ISBN:		1573-4919 (Electronic) 0300-8177 (Linking)
Abstract:		"Bovine tuberculosis (bTB) is an infectious disease with significant impact on animal health, public health and international trade. Standard bTB screening in live cattle consists in injecting tuberculin and measuring the swelling at the place of injection few days later. This procedure is expensive, time-consuming, logistically challenging, and is not conclusive before performing confirmatory tests and additional analysis. The analysis of the volatile organic compounds (VOCs) emitted by non-invasive biological samples can provide an alternative diagnostic approach suitable for bTB screening. In the present study, we analyzed VOC samples emitted through the breath, feces and skin of 18 cows diagnosed with bTB from three farms from Romania, as well as of 27 negative cows for bTB from the same farms. Analytical studies employing gas chromatography coupled to mass spectrometry revealed 80 VOCs emitted through the breath, 200 VOCs released by feces, and 80 VOCs emitted through the skin. Statistical analysis of these compounds allowed the identification of 3 tentative breath VOC biomarkers (acetone; 4-methyldecane; D-limonene), 9 tentative feces VOC biomarkers (toluene; [(1,1-dimethylethyl)thio]acetic acid; alpha-thujene; camphene; phenol; o-cymene; 3-(1,1-dimethylethyl)-2,2,4,4-tetramethyl-3-pentanol; 2,5-dimethylhexane-2,5-dihydroperoxide; 2,4-di-tert-butylphenol), and 3 tentative skin VOC biomarkers (ammonia; 1-methoxy-2-propanol; toluene). The possible pathway of these volatile biomarkers is discussed"
Keywords:		Bovine tuberculosis Breath Feces Gc-ms Skin Volatile biomarkers;
Notes:		"PublisherBrebu, Mihai Simion, Violeta Elena Andronie, Viorel Jaimes-Mogollon, Aylen Lisset Beleno-Saenz, Kelvin de Jesus Ionescu, Florina Welearegay, Tesfalem Geremariam Suschinel, Raluca de Lema, Jose Bruno Ionescu, Radu eng 777832/H2020 Marie Sklodowska-Curie Actions/ 857418/HORIZON EUROPE Widening participation and spreading excellence/ Netherlands 2023/02/26 Mol Cell Biochem. 2023 Feb 25. doi: 10.1007/s11010-023-04676-5"