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Nat Biotechnol

Title:		Yeast surface display for screening combinatorial polypeptide libraries
Author(s):		Boder ET; Wittrup KD;
Address:		"Department of Chemical Engineering, University of Illinois, Urbana 61801, USA"
Journal Title:		Nat Biotechnol
Year:		1997
Volume:		15
Issue:		6
Page Number:		553 - 557
DOI:		10.1038/nbt0697-553
ISSN/ISBN:		1087-0156 (Print) 1087-0156 (Linking)
Abstract:		"Display on the yeast cell wall is well suited for engineering mammalian cell-surface and secreted proteins (e.g., antibodies, receptors, cytokines) that require endoplasmic reticulum-specific post-translational processing for efficient folding and activity. C-terminal fusion to the Aga2p mating adhesion receptor of Saccharomyces cerevisiae has been used for the selection of scFv antibody fragments with threefold decreased antigen dissociation rate from a randomly mutated library. A eukaryotic host should alleviate expression biases present in bacterially propagated combinatorial libraries. Quantitative flow cytometric analysis enables fine discrimination of kinetic parameters for protein binding to soluble ligands"
Keywords:		"Animals Cloning, Molecular Endoplasmic Reticulum/metabolism Escherichia coli Flow Cytometry Gene Expression Genomic Library Immunoglobulin Fragments/*biosynthesis/chemistry Kinetics Mammals Mating Factor Membrane Fusion Mutagenesis *Peptide Biosynthesis *;"
Notes:		"MedlineBoder, E T Wittrup, K D eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 1997/06/01 Nat Biotechnol. 1997 Jun; 15(6):553-7. doi: 10.1038/nbt0697-553"