| Title: | Demonstration of bioprocess factors optimization for enhanced mono-rhamnolipid production by a marine Pseudomonas guguanensis |
| Author(s): | C RK; R LS; D A; V S; Vasudevan V; Krishnan MEG; |
| Address: | "Department of Biotechnology, Sri Ramachandra University, Porur, Chennai 600 116, India. Central Research Facility (CRF), Sri Ramachandra University, Porur, Chennai 600 116, India. Aquatic Animal Health and Environment Division, Central Institute of Brackishwater Aquaculture (CIBA), Indian Council of Agricultural Research (ICAR), 75, Santhome High Road, Raja Annamalai Puram, Chennai 600 028, India. PG & Research Department of Chemistry, Pachaiyappa's College, Chennai 600 030, India. Department of Biotechnology, Sri Ramachandra University, Porur, Chennai 600 116, India. Electronic address: drelizabethrajesh@sriramachandra.edu.in" |
| DOI: | 10.1016/j.ijbiomac.2017.10.186 |
| ISSN/ISBN: | 1879-0003 (Electronic) 0141-8130 (Linking) |
| Abstract: | "We identified that Pseudomonas guguanensis produced macromolecular mono-rhamnolipid (1264.52?ª+Da) upon sensing n-hexadecane/diesel/kerosene from its surroundings. Permutation experiments were done to improve the laboratory-scale mono-rhamnolipid production (ie, a three-fold increase) using RSM validation. Consequently, maximal mono-rhamnolipids production [40-50?ª+mg/L] and emulsification abilities [65-70%] were encountered on day 8 using vegetable oil, peptone?ª++?ª+yeast extract. EI(24) values for the rhamnolipids were found to be 78+/-1.75% at 12.5?ª+mg/?ª+mL. Production and secretion of rhamnolipids were accompanied by aggregation of cells at day 6 as pictured in SEM. Pure monorhamnolipids of P. guguanensis was found to lower the surface tension of water to 32.98+/-0.3?ª+mN/m than the crude and CFSs of P. aeruginosa indicating efficient activity. Utilization and subsequent removal of hexadecane was 77.2% and the breakdown products were fatty acids [decanoic, hexadecanoic, octadecanoic acids and methyl stearates] as signified in Head-space GC-MS. The breakdown products of hexadecane are also present in the synthesized rhamnolipids suggesting their biosynthetic role. Rapid degradation of hexadecane, diesel and kerosene by this emulsifier combined with non-pathogenic trait of P. guguanensis identifies this organism as a viable option to remove n-alkanes from aquatic environments" |
| Keywords: | Aquatic Organisms/isolation & purification/*metabolism/ultrastructure Biotransformation Decanoates/chemistry Emulsifying Agents/chemistry/metabolism Environmental Pollution Gas Chromatography-Mass Spectrometry Hydrocarbons/metabolism Molecular Structure M; |
| Notes: | "MedlineC, RamyaDevi K R, Lakshmi Sundaram D, Asha V, Sivamurugan Vasudevan, Vidya Krishnan, Mary Elizabeth Gnanambal eng Netherlands 2017/12/07 Int J Biol Macromol. 2018 Mar; 108:531-540. doi: 10.1016/j.ijbiomac.2017.10.186. Epub 2017 Dec 5" |
