| Title: | Jugular arginine infusion relieves lipopolysaccharide-triggered inflammatory stress and improves immunity status of lactating dairy cows |
| Author(s): | Zhao FF; Wu TY; Wang HR; Ding LY; Ahmed G; Li HW; Tian W; Shen YZ; |
| Address: | "Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. Bright Farming Co. Ltd., Shanghai 200436, China. Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. Electronic address: hrwang@yzu.edu.cn" |
| ISSN/ISBN: | 1525-3198 (Electronic) 0022-0302 (Linking) |
| Abstract: | "The objective of this study was to evaluate the effects of jugular l-Arg infusion on performance and immune function during lipopolysaccharide (LPS)-induced inflammation of lactating dairy cows. Eight Holstein cows (multiparous, 608.8 +/- 31.5 kg) at mid-lactation were randomly assigned to 5-d jugular infusions of control (saline), Arg (3 g/h), LPS (0.033 mug/kg per h), and LPS + Arg (0.033 mug/kg per h of LPS and 3 g/h of Arg) in a replicated 4 x 4 Latin square design with 4 infusion periods separated by 10-d noninfusion periods. Jugular solutions of saline, Arg, LPS, and LPS + Arg were continuously infused using peristaltic pumps for approximately 6 h/d during infusion periods. Milk yield was measured on each day of the infusion period. Milk samples were obtained on the last 2 d of each infusion period, and blood samples were obtained on the last day of each infusion period before infusion (0 h) and at 3 and 6 h. We found that the jugular LPS infusion significantly increased serum concentrations of IL-1beta, IL-6, tumor necrosis factor, inducible nitric oxide synthase, and lipopolysaccharide binding protein, whereas Arg attenuated the increase in IL-6 and inducible nitric oxide synthase levels and tended to decrease the lipopolysaccharide binding protein level. Arginine alleviated the decrease in dry matter intake and milk fat yield and the increase of somatic cell count induced by LPS. Total casein in milk was decreased during the LPS-induced inflammation period, and jugular Arg infusion significantly increased the content of total casein. In contrast, lactalbumin in milk increased during the LPS-induced inflammation period, whereas jugular Arg infusion significantly decreased the content of lactalbumin. The concentrations of plasma Gly, Thr, Ile, Leu, Arg, Phe, and total free AA were significantly decreased by LPS treatment, but Arg attenuated this tendency. These results indicated that jugular Arg infusion (18 g/d) has protective effects on relieving inflammatory stress and improving immunity status triggered by LPS. In conclusion, Arg could attenuate inflammatory stress and improve milk performance of lactating dairy cows. This protective effect may be due to the ability of Arg to suppress LPS effects and improve immunity status" |
| Keywords: | Animal Nutritional Physiological Phenomena Animals Arginine/*administration & dosage Caseins Cattle/*immunology/physiology Diet/veterinary Female *Lactation Lipopolysaccharides/*immunology Milk arginine dairy cow immunity inflammatory stress lipopolysacch; |
| Notes: | "MedlineZhao, F F Wu, T Y Wang, H R Ding, L Y Ahmed, Gulzar Li, H W Tian, W Shen, Y Z eng 2018/04/09 J Dairy Sci. 2018 Jul; 101(7):5961-5970. doi: 10.3168/jds.2017-13850. Epub 2018 Apr 5" |
