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Pest Manag Sci

Title:		Functional differentiation of two general-odorant binding proteins in Hyphantria cunea (Drury) (Lepidoptera: Erebidae)
Author(s):		Zhang X; Purba ER; Sun J; Zhang QH; Dong SL; Zhang YN; He P; Mang D; Zhang L;
Address:		"Anhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, China. Education Ministry, Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, China. Structural Cellular Biology Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, Japan. College of Life Science, Hebei University, Baoding, China. Sterling International, Inc., Spokane, WA, USA. College of Life Sciences, Huaibei Normal University, Huaibei, China. State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, China. Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan"
Journal Title:		Pest Manag Sci
Year:		2023
Volume:		20230518
Issue:		9
Page Number:		3312 - 3325
DOI:		10.1002/ps.7515
ISSN/ISBN:		1526-4998 (Electronic) 1526-498X (Linking)
Abstract:		"BACKGROUND: General odor-binding proteins (GOBPs) play critical roles in insect olfactory recognition of sex pheromones and plant volatiles. Therefore, the identification of GOBPs in Hyphantria cunea (Drury) based on their characterization to pheromone components and plant volatiles is remain unknown. RESULTS: In this study, two H. cunea (HcunGOBPs) genes were cloned, and their expression profiles and odorant binding characteristics were systematically analyzed. Firstly, the tissue expression study showed that both HcunGOBP1 and HcunGOBP2 were highly expressed in the antennae of both sexes, indicating their potential involvement in the perception of sex pheromones. Secondly, these two HcunGOBPs genes were expressed in Escherichia coli and ligand binding assays were used to assess the binding affinities to its sex pheromone components including two aldehydes and two epoxides, and some plant volatiles. HcunGOBP2 showed high binding affinities to two aldehyde components (Z9, Z12, Z15-18Ald and Z9, Z12-18Ald), and showed low binding affinities to two epoxide components (1, Z3, Z6-9S, 10R-epoxy-21Hy and Z3, Z6-9S, 10R-epoxy-21Hy), whereas HcunGOBP1 showed weak but significant binding to all four sex pheromone components. Furthermore, both HcunGOBPs demonstrated variable binding affinities to the plant volatiles tested. Thirdly, in silico studies of HcunGOBPs utilized homology, structure modeling, and molecular docking revealed critical hydrophobic residues might be involved in the binding of HcunGOBPs to their sex pheromone components and plant volatiles. CONCLUSION: Our study suggests that these two HcunGOBPs may serve as potential targets for future studies of HcunGOBPs ligand binding, providing insight in the mechanism of olfaction in H. cunea. (c) 2023 Society of Chemical Industry"
Keywords:		"Animals Female Male *Lepidoptera *Sex Attractants/chemistry Odorants Ligands Molecular Docking Simulation Insect Proteins/metabolism *Moths/genetics/metabolism *Receptors, Odorant/chemistry Gobp Hyphantria cunea ligand binding assay olfaction plant volati;"
Notes:		"MedlineZhang, Xiaoqing Purba, Endang R Sun, Jing Zhang, Qing-He Dong, Shuang-Lin Zhang, Ya-Nan He, Peng Mang, Dingze Zhang, Longwa eng Anhui Provincial Key Research and Development Program (202201020010)/ Hebei Natural Science Foundation (C2022201042)/ National Key Research and Development Program (2018YFC1200400)/ National Key Research and Development Program (2018YFC1200400), Natural Science Foundation of Anhui province, China (1508085SMC216)/ National Key Research and Development Program of China (2022YFC2601000)/ This project was funded by the National Natural Science Foundation of China (31870640)/ England 2023/04/27 Pest Manag Sci. 2023 Sep; 79(9):3312-3325. doi: 10.1002/ps.7515. Epub 2023 May 18"