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J Chem Ecol

Title:		"Molecular characterization, expression pattern, and ligand-binding property of three odorant binding protein genes from Dendrolimus tabulaeformis"
Author(s):		Zhang S; Zhang Z; Wang H; Kong X;
Address:		"Key Laboratory of Forest Protection, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, State Forestry Administration, No.1 Dongxiaofu, Haidian, Beijing, China"
Journal Title:		J Chem Ecol
Year:		2014
Volume:		20140412
Issue:		4
Page Number:		396 - 406
DOI:		10.1007/s10886-014-0412-6
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Print) 0098-0331 (Linking)
Abstract:		"Odorant binding proteins (OBPs) play important roles in insect olfactory processes. The Chinese pine caterpillar moth, Dendrolimus tabulaeformis (Lepidoptera, Lasiocampidae) is a serious economic pest in China, and the pheromones of this species have been identified to monitor their presence. However, the molecular mechanisms by which D. tabulaeformis perceive pheromones and host volatiles remain unknown. In this study, we identified and characterized three new OBPs, including one pheromone binding protein (PBP1) and two general odor binding proteins (GOBPs), from antennal cDNA of D. tabulaeformis. The deduced amino acid sequences of DtabPBP1, DtabGOBP1, and DtabGOBP2 revealed mature proteins of 140, 147, and 140 amino acids, respectively. Each has six cysteine residues in conserved positions relative to other known OBPs. Amino-acid alignments indicated that the two GOBPs are more conserved (DtabGOBP1 is 52.9-67.4 % identical to orthologs from other Lepidoptera, and DtabGOBP2 is 55.2-81.8 % identical) than the PBP (32.5-46.0 %). Real-time PCR indicated tissue- and sex-specific expression patterns of the three genes. DtabPBP1 was mainly expressed in the antennae of males, whereas female antennae had only 1.09 % the expression in male antennae. Both DtabGOBP1 and DtabGOBP2 were more highly expressed in antennae than in other tissues, while DtabGOBP1 was more abundant in male antennae and DtabGOBP2 in female antennae. In addition, the binding specificities of the three proteins were investigated, and all three OBPs exhibited high binding affinities for the pheromone component (5Z,7E)-5,7-dodecadien-1-yl propionate (Z5,E7-12:OPr). This suggests a role in binding pheromone for GOBPs, as well as PBP1, in D. tabulaeformis"
Keywords:		"Amino Acid Sequence Animals Base Sequence Carrier Proteins/chemistry/*genetics/metabolism DNA, Complementary/genetics/metabolism Female Gene Expression Profiling *Gene Expression Regulation Insect Proteins/chemistry/*genetics/metabolism Male Molecular Seq;"
Notes:		"MedlineZhang, Sufang Zhang, Zhen Wang, Hongbin Kong, Xiangbo eng Research Support, Non-U.S. Gov't 2014/04/15 J Chem Ecol. 2014 Apr; 40(4):396-406. doi: 10.1007/s10886-014-0412-6. Epub 2014 Apr 12"