| Title: | Demethylation and microRNA differential expression regulate plasma-induced improvement of chicken sperm quality |
| Author(s): | Zhang JJ; Chandimali N; Kim N; Kang TY; Kim SB; Kim JS; Wang XZ; Kwon T; Jeong DK; |
| Address: | "Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, 400715, P.R. China. Laboratory of Animal Genetic Engineering and Stem Cell Biology, Department of Advanced Convergence Technology and Science, Jeju National University, Jeju, 63243, Republic of Korea. Plasma Technology Research Center, National Fusion Research Institute, Gunsan-si, Jeollabuk-Do, 54004, Republic of Korea. Primate Resources Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Jeonbuk, 56216, Republic of Korea. Chongqing Key Laboratory of Forage and Herbivore, College of Animal Science and Technology, Southwest University, Chongqing, 400715, P.R. China. xianzhong_wang@aliyun.com. Primate Resources Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Jeonbuk, 56216, Republic of Korea. kwon@kribb.re.kr. Laboratory of Animal Genetic Engineering and Stem Cell Biology, Department of Advanced Convergence Technology and Science, Jeju National University, Jeju, 63243, Republic of Korea. ngejeong@gmail.com" |
| DOI: | 10.1038/s41598-019-45087-1 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "The sperm quality is a vital economical requisite of poultry production. Our previous study found non-thermal dielectric barrier discharge plasma exposure on fertilized eggs could increase the chicken growth and the male reproduction. However, it is unclear how plasma treatment regulates the reproductive capacity in male chickens. In this study, we used the optimal plasma treatment condition (2.81 W for 2 min) which has been applied on 3.5-day-incubated fertilized eggs in the previous work and investigated the reproductive performance in male chickens aged at 20 and 40 weeks. The results showed that plasma exposure increased sperm count, motility, fertility rate, and fertilization period of male chickens. The sperm quality-promoting effect of plasma treatment was regulated by the significant improvements of adenosine triphosphate production and testosterone level, and by the modulation of reactive oxygen species balance and adenosine monophosphate-activated protein kinase and mammalian target of rapamycin pathway in the spermatozoa. Additionally, the plasma effect suggested that DNA demethylation and microRNA differential expression (a total number of 39 microRNAs were up-regulated whereas 53 microRNAs down-regulated in the testis) regulated the increases of adenosine triphosphate synthesis and testosterone level for promoting the chicken sperm quality. This finding might be beneficial to elevate the fertilization rate and embryo quality for the next generation in poultry breeding" |
| Keywords: | Adenosine Triphosphate/blood Animals *Chickens Male MicroRNAs/*genetics Reactive Oxygen Species/blood Sperm Count Sperm Motility Spermatozoa/*cytology Testis/*physiology Testosterone/blood; |
| Notes: | "MedlineZhang, Jiao Jiao Chandimali, Nisansala Kim, Nameun Kang, Tae Yoon Kim, Seong Bong Kim, Ji Su Wang, Xian Zhong Kwon, Taeho Jeong, Dong Kee eng Research Support, Non-U.S. Gov't England 2019/06/22 Sci Rep. 2019 Jun 20; 9(1):8865. doi: 10.1038/s41598-019-45087-1" |
