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PLoS One

Title:		"Antennal transcriptome analysis and comparison of chemosensory gene families in two closely related noctuidae moths, Helicoverpa armigera and H. assulta"
Author(s):		Zhang J; Wang B; Dong S; Cao D; Dong J; Walker WB; Liu Y; Wang G;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; Education Ministry Key Laboratory of Integrated Management of Crop Disease and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Education Ministry Key Laboratory of Integrated Management of Crop Disease and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China. College of Forestry, Henan University of Science and Technology, Luoyang, 471003, China. Swedish University of Agricultural Sciences, Department of Plant Protection Biology, Chemical Ecology Research Group, Alnarp, Sweden"
Journal Title:		PLoS One
Year:		2015
Volume:		20150206
Issue:		2
Page Number:		e0117054 -
DOI:		10.1371/journal.pone.0117054
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"To better understand the olfactory mechanisms in the two lepidopteran pest model species, the Helicoverpa armigera and H. assulta, we conducted transcriptome analysis of the adult antennae using Illumina sequencing technology and compared the chemosensory genes between these two related species. Combined with the chemosensory genes we had identified previously in H. armigera by 454 sequencing, we identified 133 putative chemosensory unigenes in H. armigera including 60 odorant receptors (ORs), 19 ionotropic receptors (IRs), 34 odorant binding proteins (OBPs), 18 chemosensory proteins (CSPs), and 2 sensory neuron membrane proteins (SNMPs). Consistent with these results, 131 putative chemosensory genes including 64 ORs, 19 IRs, 29 OBPs, 17 CSPs, and 2 SNMPs were identified through male and female antennal transcriptome analysis in H. assulta. Reverse Transcription-PCR (RT-PCR) was conducted in H. assulta to examine the accuracy of the assembly and annotation of the transcriptome and the expression profile of these unigenes in different tissues. Most of the ORs, IRs and OBPs were enriched in adult antennae, while almost all the CSPs were expressed in antennae as well as legs. We compared the differences of the chemosensory genes between these two species in detail. Our work will surely provide valuable information for further functional studies of pheromones and host volatile recognition genes in these two related species"
Keywords:		"Animals Arthropod Antennae/*metabolism Female Gene Expression Profiling/methods Gene Expression Regulation/*physiology Insect Proteins/*biosynthesis Male Moths Receptors, Odorant/*biosynthesis Transcriptome/*physiology;"
Notes:		"MedlineZhang, Jin Wang, Bing Dong, Shuanglin Cao, Depan Dong, Junfeng Walker, William B Liu, Yang Wang, Guirong eng Research Support, Non-U.S. Gov't 2015/02/07 PLoS One. 2015 Feb 6; 10(2):e0117054. doi: 10.1371/journal.pone.0117054. eCollection 2015"