| Title: | Enhanced non-invasive respiratory sampling from bottlenose dolphins for breath metabolomics measurements |
| Author(s): | Zamuruyev KO; Aksenov AA; Baird M; Pasamontes A; Parry C; Foutouhi S; Venn-Watson S; Weimer BC; Delplanque JP; Davis CE; |
| Address: | "Department of Mechanical and Aerospace Engineering, One Shields Avenue, University of California, Davis, Davis, CA 95616, USA" |
| DOI: | 10.1088/1752-7155/10/4/046005 |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Print) 1752-7155 (Linking) |
| Abstract: | "Chemical analysis of exhaled breath metabolites is an emerging alternative to traditional clinical testing for many physiological conditions. The main advantage of breath analysis is its inherent non-invasive nature and ease of sample collection. Therefore, there exists a great interest in further development of this method for both humans and animals. The physiology of cetaceans is exceptionally well suited for breath analysis due to their explosive breathing behavior and respiratory tract morphology. At the present time, breath analysis in cetaceans has very limited practical applications, in large part due to lack of widely adopted sampling device(s) and methodologies that are well-standardized. Here, we present an optimized design and the operating principles of a portable apparatus for reproducible collection of exhaled breath condensate from small cetaceans, such as bottlenose dolphins (Tursiops truncatus). The device design is optimized to meet two criteria: standardized collection and preservation of information-rich metabolomic content of the biological sample, and animal comfort and ease of breath sample collection. The intent is to furnish a fully-benchmarked technology that can be widely adopted by researchers and conservationists to spur further developments of breath analysis applications for marine mammal health assessments" |
| Keywords: | Animals Bottle-Nosed Dolphin/*metabolism Breath Tests/*methods Exhalation Hot Temperature Humans Metabolomics/*methods *Respiration Specimen Handling Volatile Organic Compounds/analysis; |
| Notes: | "MedlineZamuruyev, Konstantin O Aksenov, Alexander A Baird, Mark Pasamontes, Alberto Parry, Celeste Foutouhi, Soraya Venn-Watson, Stephanie Weimer, Bart C Delplanque, Jean-Pierre Davis, Cristina E eng U01 EB0220003-01/EB/NIBIB NIH HHS/ U01 EB022003/EB/NIBIB NIH HHS/ P42ES004699/ES/NIEHS NIH HHS/ T32 HL007013/HL/NHLBI NIH HHS/ T32 HL07013/HL/NHLBI NIH HHS/ P42 ES004699/ES/NIEHS NIH HHS/ P30 ES023513/ES/NIEHS NIH HHS/ !P30ES023513-01A1/ES/NIEHS NIH HHS/ UL1 TR000002/TR/NCATS NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. England 2016/10/01 J Breath Res. 2016 Sep 30; 10(4):046005. doi: 10.1088/1752-7155/10/4/046005" |
