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PLoS One

Title:		Conservation and lineage-specific rearrangements in the GOBP/PBP gene complex of distantly related ditrysian Lepidoptera
Author(s):		Yasukochi Y; Yang B; Fujimoto T; Sahara K; Matsuo T; Ishikawa Y;
Address:		"Institute of Agrobiological Sciences, NARO, Tsukuba, Ibaraki, Japan. Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan. Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan"
Journal Title:		PLoS One
Year:		2018
Volume:		20180209
Issue:		2
Page Number:		e0192762 -
DOI:		10.1371/journal.pone.0192762
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"General odorant binding proteins (GOBPs) and pheromone binding proteins (PBPs) form a monophyletic subfamily of insect odorant binding proteins (OBPs) specific for Lepidoptera, butterflies and moths. The GOBP/PBP genes include six subgroups (GOBP1-2, PBP-A-D) previously reported to form a complex arrayed in a conserved order in representative moths (superfamily Bombycoidea) and butterflies (Nymphalidae). Although our knowledge of lepidopteran genomes has increased greatly recently, the structure of the GOBP/PBP complex has been studied only for species that represent limited lineages of the highly diverged Ditrysia. To understand the evolution of this functionally important gene complex, we determined 69-149 kb genomic sequences that include GOBP2 and five PBP genes in three Ostrinia moths (Pyraloidea), O. nubilalis, O. furnacalis, and O. latipennis, using bacterial artificial chromosome (BAC) and fosmid clones. The structure of the GOBP2/PBP gene cluster was well conserved despite the different sex pheromone composition utilized by the three moths. Five expressed PBP genes in Ostrinia moths were the result of two duplications of PBP-A genes. Surprisingly, an allele containing a fusion gene between tandemly arrayed PBP-A genes was observed in O. nubilalis. We also revealed duplication and intra-chromosomal translocation of the GOBP1 gene in P. xylostella by fluorescence in situ hybridization (FISH) analysis. Additionally, we compared the structure of the GOBP/PBP gene complex of seventeen species covering six superfamilies and twelve families of the lepidopteran clade, Ditrysia, and found the gene order was basically conserved despite the frequent occurrence of lineage-specific gains, losses, inversions and translocations of these genes, compared with their neighboring genes. Our findings support the hypothesis that the structure of the GOBP/PBP gene complex was already established in the common ancestor of Ditrysia"
Keywords:		"Animals Carrier Proteins/*genetics Chromosomes, Artificial, Bacterial In Situ Hybridization, Fluorescence Insect Proteins/*genetics Lepidoptera/*genetics Multigene Family Phylogeny Receptors, Odorant/*genetics Transcription, Genetic Translocation, Genetic;"
Notes:		"MedlineYasukochi, Yuji Yang, Bin Fujimoto, Toshiaki Sahara, Ken Matsuo, Takashi Ishikawa, Yukio eng Research Support, Non-U.S. Gov't 2018/02/10 PLoS One. 2018 Feb 9; 13(2):e0192762. doi: 10.1371/journal.pone.0192762. eCollection 2018"