« Previous AbstractAroma dynamic characteristics during the process of variable-temperature final firing of Congou black tea by electronic nose and comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry    Next AbstractThe exposure risk of typical VOCs to the human beings via inhalation based on the respiratory deposition rates by proton transfer reaction-time of flight-mass spectrometer »

Anal Bioanal Chem

Title:		Derivatization gas chromatography negative chemical ionization mass spectrometry for the analysis of trace organic pollutants and their metabolites in human biological samples
Author(s):		Yang Y; Lin M; Tang J; Ma S; Yu Y;
Address:		"Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou, 510006, Guangdong, China. Synergy Innovation Institute of GDUT, Shantou, 515100, Guangdong, China. Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou, 510006, Guangdong, China. mast@gdut.edu.cn. Synergy Innovation Institute of GDUT, Shantou, 515100, Guangdong, China. mast@gdut.edu.cn"
Journal Title:		Anal Bioanal Chem
Year:		2020
Volume:		20200618
Issue:		25
Page Number:		6679 - 6690
DOI:		10.1007/s00216-020-02762-x
ISSN/ISBN:		1618-2650 (Electronic) 1618-2642 (Linking)
Abstract:		"Gas chromatography negative chemical ionization mass spectrometry (GC-NCI-MS) is a preferred instrumental approach for the trace and ultra-trace analysis of various toxic organics and their metabolites in human biological fluids. Specifically, the method has played an important role in the highly sensitive and specific quantitative detection of persistent highly halogenated compounds in environmental matrices and biota during the past few decades. However, for the analysis of toxic metabolites with active hydrogen atoms, such as acids, alcohols, and phenolic compounds, from biological matrixes or organics without electronegative atoms or groups, a derivatization step is often needed prior to GC analysis. Such derivatization aims to change the properties of targets to improve their separation, increase their volatility, and enhance the sensitivity of instrumental detection. This review summarizes three derivatization strategies commonly used for GC methods, i.e., alkylation, silylation, and acylation, together with their application combined with GC-NCI-MS for the high sensitivity analysis of toxic organic metabolites in the human body. The advantages and disadvantages of each derivatization method and potential directions for future applications are discussed. Given the broad variety of applications as well as the compound-specific sensitivity for the ultra-trace analysis of target xenobiotics in human biological fluids, subsequent studies are required to develop convenient, faster derivatization procedures and reagents better suited for routine analysis. Graphical abstract"
Keywords:		Alkylation Body Fluids/*chemistry Environmental Exposure Environmental Pollutants/*analysis Gas Chromatography-Mass Spectrometry/*methods Humans Indicators and Reagents/chemistry Limit of Detection Organic Chemicals/*analysis Acylation Derivatization Meta;
Notes:		"MedlineYang, Yan Lin, Meiqing Tang, Jian Ma, Shengtao Yu, Yingxin eng 41991310/National Natural Science Foundation of China/ 2017BT01Z032/Local Innovative and Research Teams Project of the Guangdong Pearl River Talents Program/ Review Germany 2020/06/20 Anal Bioanal Chem. 2020 Oct; 412(25):6679-6690. doi: 10.1007/s00216-020-02762-x. Epub 2020 Jun 18"