| Title: | Long-lasting memory of jasmonic acid-dependent immunity requires DNA demethylation and ARGONAUTE1 |
| Author(s): | Wilkinson SW; Hannan Parker A; Muench A; Wilson RS; Hooshmand K; Henderson MA; Moffat EK; Rocha P; Hipperson H; Stassen JHM; Lopez Sanchez A; Fomsgaard IS; Krokene P; Mageroy MH; Ton J; |
| Address: | "Plants, Photosynthesis and Soil, School of Biosciences, Institute for Sustainable Food, The University of Sheffield, Sheffield, UK. samuel.wilkinson@sheffield.ac.uk. Plants, Photosynthesis and Soil, School of Biosciences, Institute for Sustainable Food, The University of Sheffield, Sheffield, UK. Department of Agroecology, Aarhus University, Slagelse, Denmark. Division for Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research (NIBIO), As, Norway. Plants, Photosynthesis and Soil, School of Biosciences, Institute for Sustainable Food, The University of Sheffield, Sheffield, UK. j.ton@sheffield.ac.uk" |
| DOI: | 10.1038/s41477-022-01313-9 |
| ISSN/ISBN: | 2055-0278 (Electronic) 2055-0278 (Linking) |
| Abstract: | "Stress can have long-lasting impacts on plants. Here we report the long-term effects of the stress hormone jasmonic acid (JA) on the defence phenotype, transcriptome and DNA methylome of Arabidopsis. Three weeks after transient JA signalling, 5-week-old plants retained induced resistance (IR) against herbivory but showed increased susceptibility to pathogens. Transcriptome analysis revealed long-term priming and/or upregulation of JA-dependent defence genes but repression of ethylene- and salicylic acid-dependent genes. Long-term JA-IR was associated with shifts in glucosinolate composition and required MYC2/3/4 transcription factors, RNA-directed DNA methylation, the DNA demethylase ROS1 and the small RNA (sRNA)-binding protein AGO1. Although methylome analysis did not reveal consistent changes in DNA methylation near MYC2/3/4-controlled genes, JA-treated plants were specifically enriched with hypomethylated ATREP2 transposable elements (TEs). Epigenomic characterization of mutants and transgenic lines revealed that ATREP2 TEs are regulated by RdDM and ROS1 and produce 21 nt sRNAs that bind to nuclear AGO1. Since ATREP2 TEs are enriched with sequences from IR-related defence genes, our results suggest that AGO1-associated sRNAs from hypomethylated ATREP2 TEs trans-regulate long-lasting memory of JA-dependent immunity" |
| Keywords: | *Arabidopsis Proteins/genetics/metabolism DNA Demethylation Protein-Tyrosine Kinases/genetics/metabolism/pharmacology Proto-Oncogene Proteins/genetics/metabolism/pharmacology *Arabidopsis/metabolism Cyclopentanes/metabolism Oxylipins/metabolism RNA/metabo; |
| Notes: | "MedlineWilkinson, S W Hannan Parker, A Muench, A Wilson, R S Hooshmand, K Henderson, M A Moffat, E K Rocha, P S C F Hipperson, H Stassen, J H M Lopez Sanchez, A Fomsgaard, I S Krokene, P Mageroy, M H Ton, J eng Promo158/Deutscher Akademischer Austauschdienst (German Academic Exchange Service)/ 249920/Norges Forskningsrad (Research Council of Norway)/ 249958/F20/Norges Forskningsrad (Research Council of Norway)/ 309944/EC | EC Seventh Framework Programm | FP7 Ideas: European Research Council (FP7-IDEAS-ERC - Specific Programme: 'Ideas' Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013))/ 824985/EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)/ RL-2012-042/Leverhulme Trust/ BB/P006698/1/RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)/ Research Support, Non-U.S. Gov't England 2023/01/06 Nat Plants. 2023 Jan; 9(1):81-95. doi: 10.1038/s41477-022-01313-9. Epub 2023 Jan 5" |
