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Nat Commun

Title:		Identification of a plastidial phenylalanine exporter that influences flux distribution through the phenylalanine biosynthetic network
Author(s):		Widhalm JR; Gutensohn M; Yoo H; Adebesin F; Qian Y; Guo L; Jaini R; Lynch JH; McCoy RM; Shreve JT; Thimmapuram J; Rhodes D; Morgan JA; Dudareva N;
Address:		"Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, Indiana 47907-2063, USA. Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Dr, West Lafayette, Indiana 47907-2010, USA. School of Chemical Engineering, Purdue University, 480 Stadium Mall Dr, West Lafayette, Indiana 47907-2100, USA. Bioinformatics Core, Purdue University, 155 South Grant Street, West Lafayette, Indiana 47907, USA"
Journal Title:		Nat Commun
Year:		2015
Volume:		20150910
Issue:
Page Number:		8142 -
DOI:		10.1038/ncomms9142
ISSN/ISBN:		2041-1723 (Electronic) 2041-1723 (Linking)
Abstract:		"In addition to proteins, L-phenylalanine is a versatile precursor for thousands of plant metabolites. Production of phenylalanine-derived compounds is a complex multi-compartmental process using phenylalanine synthesized predominantly in plastids as precursor. The transporter(s) exporting phenylalanine from plastids, however, remains unknown. Here, a gene encoding a Petunia hybrida plastidial cationic amino-acid transporter (PhpCAT) functioning in plastidial phenylalanine export is identified based on homology to an Escherichia coli phenylalanine transporter and co-expression with phenylalanine metabolic genes. Radiolabel transport assays show that PhpCAT exports all three aromatic amino acids. PhpCAT downregulation and overexpression result in decreased and increased levels, respectively, of phenylalanine-derived volatiles, as well as phenylalanine, tyrosine and their biosynthetic intermediates. Metabolic flux analysis reveals that flux through the plastidial phenylalanine biosynthetic pathway is reduced in PhpCAT RNAi lines, suggesting that the rate of phenylalanine export from plastids contributes to regulating flux through the aromatic amino-acid network"
Keywords:		"Amino Acid Transport Systems, Basic/*metabolism Biosynthetic Pathways Escherichia coli Metabolic Flux Analysis Petunia Phenylalanine/*metabolism Plant Proteins/*metabolism Plants, Genetically Modified Plastids/*metabolism RNA Interference Real-Time Polyme;"
Notes:		"MedlineWidhalm, Joshua R Gutensohn, Michael Yoo, Heejin Adebesin, Funmilayo Qian, Yichun Guo, Longyun Jaini, Rohit Lynch, Joseph H McCoy, Rachel M Shreve, Jacob T Thimmapuram, Jyothi Rhodes, David Morgan, John A Dudareva, Natalia eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2015/09/12 Nat Commun. 2015 Sep 10; 6:8142. doi: 10.1038/ncomms9142"