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Nature

Title:		Genetic interactions between polymorphisms that affect gene expression in yeast
Author(s):		Brem RB; Storey JD; Whittle J; Kruglyak L;
Address:		"Program in Computational Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, M2-B876, Seattle, Washington 98109, USA. rbrem@fhcrc.org"
Journal Title:		Nature
Year:		2005
Volume:		436
Issue:		7051
Page Number:		701 - 703
DOI:		10.1038/nature03865
ISSN/ISBN:		1476-4687 (Electronic) 0028-0836 (Print) 0028-0836 (Linking)
Abstract:		"Interactions between polymorphisms at different quantitative trait loci (QTLs) are thought to contribute to the genetics of many traits, and can markedly affect the power of genetic studies to detect QTLs. Interacting loci have been identified in many organisms. However, the prevalence of interactions, and the nucleotide changes underlying them, are largely unknown. Here we search for naturally occurring genetic interactions in a large set of quantitative phenotypes--the levels of all transcripts in a cross between two strains of Saccharomyces cerevisiae. For each transcript, we searched for secondary loci interacting with primary QTLs detected by their individual effects. Such locus pairs were estimated to be involved in the inheritance of 57% of transcripts; statistically significant pairs were identified for 225 transcripts. Among these, 67% of secondary loci had individual effects too small to be significant in a genome-wide scan. Engineered polymorphisms in isogenic strains confirmed an interaction between the mating-type locus MAT and the pheromone response gene GPA1. Our results indicate that genetic interactions are widespread in the genetics of transcript levels, and that many QTLs will be missed by single-locus tests but can be detected by two-stage tests that allow for interactions"
Keywords:		"Crosses, Genetic GTP-Binding Protein alpha Subunits/genetics/metabolism GTP-Binding Protein alpha Subunits, Gq-G11 Gene Expression Regulation, Fungal/*genetics Genes, Fungal/genetics Genes, Mating Type, Fungal Genome, Fungal Haploidy Mutation/genetics Phe;"
Notes:		"MedlineBrem, Rachel B Storey, John D Whittle, Jacqueline Kruglyak, Leonid eng R01 HG002913/HG/NHGRI NIH HHS/ R37 MH059520/MH/NIMH NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. England 2005/08/05 Nature. 2005 Aug 4; 436(7051):701-3. doi: 10.1038/nature03865"