| Title: | Effect of Thidiazuron on Terpene Volatile Constituents and Terpenoid Biosynthesis Pathway Gene Expression of Shine Muscat (Vitis labrusca x V. vinifera) Grape Berries |
| Author(s): | Wang W; Khalil-Ur-Rehman M; Wei LL; Nieuwenhuizen NJ; Zheng H; Tao JM; |
| Address: | "College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. The New Zealand Institute for Plant and Food Research Ltd. (PFR), Private Bag 92169, Auckland 1142, New Zealand" |
| DOI: | 10.3390/molecules25112578 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Volatile compounds are considered to be essential for the flavor and aroma quality of grapes. Thidiazuron (TDZ) is a commonly used growth regulator in grape cultivation that stimulates larger berries and prevents fruit drop. This study was conducted to investigate the effect of TDZ on the production of aroma volatiles and to identify the key genes involved in the terpene biosynthesis pathways that are affected by this compound. Treatment with TDZ had a negative effect on the concentration of volatile compounds, especially on monoterpenes, which likely impacts the sensory characteristics of the fruit. The expression analysis of genes related to the monoterpenoid biosynthesis pathways confirmed that treatment with TDZ negatively regulated the key genes DXS1, DXS3, DXR, HDR, VvPNGer and VvPNlinNer1. Specifically, the expression levels of the aforementioned genes were down-regulated in almost all berry development stages in the TDZ-treated samples. The novel results from the present study can be used to aid in the development of food products which maintain the flavor quality and sensory characteristics of grape. Furthermore, these findings can provide the theoretical basis that can help to optimize the utilization of TDZ for the field production of grapes at a commercial scale" |
| Keywords: | "Down-Regulation Gene Expression Profiling Gene Expression Regulation, Developmental/drug effects Gene Expression Regulation, Plant/drug effects Monoterpenes/*metabolism Odorants/analysis Phenylurea Compounds/*pharmacology Plant Proteins/*genetics Thiadiaz;" |
| Notes: | "MedlineWang, Wu Khalil-Ur-Rehman, Muhammad Wei, Ling-Ling Nieuwenhuizen, Niels J Zheng, Huan Tao, Jian-Min eng 2018YFD0201300/National Key Research and Development Program of China/ CARS-29/China Agriculture Research System/ JATS [2017]274/Jiangsu Agricultural Industry Technology System/ PZCZ201723/Jiangsu Key Agricultural Project for New Cultivars Innovation/ Switzerland 2020/06/06 Molecules. 2020 Jun 2; 25(11):2578. doi: 10.3390/molecules25112578" |
