| Title: | "Effects of an allelochemical in Phaeodactylum tricornutum filtrate on Heterosigma akashiwo: Morphological, physiological and growth effects" |
| Author(s): | Wang R; Xue Q; Wang J; Tan L; Zhang Q; Zhao Y; Anderson DM; |
| Address: | "Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Shenzhen Key Laboratory for the Sustainable Use of Marine Biodiversity, Research Centre for the Oceans and Human Health, City University of Hong Kong Shenzhen Research Institute, Shenzhen 518057, China. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China. Electronic address: jtwang@ouc.edu.cn. Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, United States" |
| DOI: | 10.1016/j.chemosphere.2017.08.024 |
| ISSN/ISBN: | 1879-1298 (Electronic) 0045-6535 (Print) 0045-6535 (Linking) |
| Abstract: | "The effects of an allelochemical extracted from the culture filtrate of diatom Phaeodactylum tricornutum on the raphidophyte Heterosigma akashiwo were investigated using a series of morphological, physiological and biochemical characters. Growth experiments showed that H. akashiwo was significantly inhibited immediately after exposure to the allelochemical, with many cells rapidly dying and lysing based on microscopic observation. The effects of the allelochemical on the surviving cells were explored using Scanning Electron Microscopy (SEM) and Flow cytometry (FCM), the latter by examination of a suite of physiological parameters (membrane integrity, esterase activity, chlorophyll-a content, membrane potential). The results demonstrate that the membrane of H. akashiwo was attacked by the allelochemical directly, causing cell membrane breakage and loss of integrity. Esterase activity was the most sensitive indicator of the impacts of the allelochemical. Membrane potential and chlorophyll-a content both showed significant decreases following exposure of the Heterosigma cells to high concentrations of the allelochemical for 5 and 6 days. Both were affected, but the membrane potential response was more gradual compared to other effects. The cell size of H. akashiwo did not change compared with the control group. The surviving cells were able to continue to grow and in a few days, re-establish a successful culture, even in the presence of residual allelochemical, suggesting either development of cellular resistance, or the degradation of the chemical" |
| Keywords: | "Chlorophyll/metabolism Chlorophyll A Diatoms/*drug effects/growth & development/ultrastructure Dinoflagellida/*chemistry/growth & development Dose-Response Relationship, Drug Esterases/metabolism Flow Cytometry Membrane Potentials/drug effects Microscopy, ;" |
| Notes: | "MedlineWang, Rui Xue, Qiaona Wang, Jiangtao Tan, Liju Zhang, Qingchun Zhao, Yue Anderson, Donald M eng P01 ES021923/ES/NIEHS NIH HHS/ England 2017/08/16 Chemosphere. 2017 Nov; 186:527-534. doi: 10.1016/j.chemosphere.2017.08.024. Epub 2017 Aug 7" |
