| Title: | Ex planta phytoremediation of trichlorophenol and phenolic allelochemicals via an engineered secretory laccase |
| Author(s): | Wang GD; Li QJ; Luo B; Chen XY; |
| Address: | "National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, P.R. China" |
| ISSN/ISBN: | 1087-0156 (Print) 1087-0156 (Linking) |
| Abstract: | "Plant roots release a range of enzymes capable of degrading chemical compounds in their immediate vicinity. We present a system of phytoremediation ex planta based on the overexpression of one such enzyme, a secretory laccase. Laccases catalyze the oxidation of a broad range of phenolic compounds, including polychlorinated phenols such as 2,4,6-trichlorophenol (TCP), that are among the most hazardous and recalcitrant pollutants in the environment. We isolated a secretory laccase cDNA of LAC1, which is specifically expressed in the roots of Gossypium arboreum (cotton). Transgenic Arabidopsis thaliana plants overexpressing LAC1 exhibited enhanced resistance to several phenolic allelochemicals and TCP. The secretory laccase activity in these plants was responsible for the conversion of sinapic acid into a mono-lactone type dimer and for the transformation of TCP" |
| Keywords: | "Arabidopsis/*genetics/metabolism Biotransformation Chlorophenols/*metabolism/pharmacology Cloning, Molecular Coumaric Acids/analysis Drug Resistance Gallic Acid/*analogs & derivatives/analysis Gossypium/*enzymology/genetics Hydroxybenzoates/*metabolism/ph;" |
| Notes: | "MedlineWang, Guo-Dong Li, Qian-Jin Luo, Bin Chen, Xiao-Ya eng Research Support, Non-U.S. Gov't 2004/06/15 Nat Biotechnol. 2004 Jul; 22(7):893-7. doi: 10.1038/nbt982. Epub 2004 Jun 13" |
