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J Chromatogr Sci

Title:		Characterization of volatile components in dry chrysanthemum flowers using headspace-liquid-phase microextraction-gas chromatography
Author(s):		Wang G; Dong C; Sun YA; Xie K; Zheng H;
Address:		"Department of Applied Chemistry, Zhengzhou University of Light Industry, Zhengzhou, Henan, 450002, China. gqwang@ustc.edu"
Journal Title:		J Chromatogr Sci
Year:		2008
Volume:		46
Issue:		2
Page Number:		127 - 132
DOI:		10.1093/chromsci/46.2.127
ISSN/ISBN:		0021-9665 (Print) 0021-9665 (Linking)
Abstract:		"A headspace-liquid-phase microextraction (HS-LPME)-GC (gas chromatography) method for the characterization of volatile components in dry chrysanthemum flowers has been developed. In the proposed method, two extraction solvents, n-hexadecane and benzyl alcohol, are used for preconcentrating volatiles in the sample. A droplet of the extraction solvent is squeezed from the GC syringe and inserted in the headspace of the sample bottle with the dry flower, immersed in deionized water, and warmed in a water bath. The optimum HS-LPME parameters in terms of extraction solvent type, droplet magnitude, equilibrium (water bath) temperature, equilibrium time, extraction time, and ionic strength are achieved using GC-FID (flame ionization detection) by varying several levels of the factors that affect the HS-LPME procedure. After extraction under the optimized conditions, the extraction droplet is retracted into the syringe and injected for GC-MS (mass spectrometry) analysis. Thirty-three volatile components are extracted and identified using this HS-LPME-GC-MS method, with the aid of chemometric methods. It is shown that the volatiles in dry chrysanthemum flowers are mainly unsaturated organic compounds, such as monoterpenes, sesquiterpenes and their oxygenous derivatives, triterpenoids, and aliphatic compounds. Several representative components, in order of precedence of the retention time, are pinene (106.3 microg/g), camphene (112.7 microg/g), eucapyptol (52.1 microg/g), camphor (29.4 microg/g), borneol (4.2 microg g), bornyl acetate (67.3 microg/g), caryophyllene (0.7 microg/g), and caryophyllene oxide (20.0 microg/g). The relative standard error and detection limit of this method is 5-9% and 0.4 microg/g, respectively"
Keywords:		"Chemical Fractionation/methods Chrysanthemum/*chemistry Drugs, Chinese Herbal/*chemistry/isolation & purification Flowers/*chemistry Gas Chromatography-Mass Spectrometry/*methods Oils, Volatile/*chemistry/isolation & purification Sodium Chloride Solubilit;"
Notes:		"MedlineWang, Guoqing Dong, Chunhong Sun, Yu-an Xie, Kui Zheng, Haiyu eng Research Support, Non-U.S. Gov't 2008/03/28 J Chromatogr Sci. 2008 Feb; 46(2):127-32. doi: 10.1093/chromsci/46.2.127"