| Title: | Headspace gas chromatography-mass spectrometry in the analysis of lavender's essential oil: Optimization by response surface methodology |
| Author(s): | Virgiliou C; Zisi C; Kontogiannopoulos KN; Nakas A; Iakovakis A; Varsamis V; Gika HG; Assimopoulou AN; |
| Address: | "Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; Center for Bioanalysis & Omics (BIOMIC_AUTH), CIRI Center for Interdisciplinary Research and Innovation, Aristotle University of Thessaloniki, 57001 Thessaloniki, Greece. Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; Natural Products Research Centre of Excellence (NatPro-AUTH), Center for Interdisciplinary Research and Innovation of Aristotle University of Thessaloniki (CIRI-AUTH), 57001 Thessaloniki, Greece. School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; Center for Bioanalysis & Omics (BIOMIC_AUTH), CIRI Center for Interdisciplinary Research and Innovation, Aristotle University of Thessaloniki, 57001 Thessaloniki, Greece. Vessel Essential Oils, Neo Rysio, 57001 Thessaloniki, Greece. School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; Center for Bioanalysis & Omics (BIOMIC_AUTH), CIRI Center for Interdisciplinary Research and Innovation, Aristotle University of Thessaloniki, 57001 Thessaloniki, Greece. Electronic address: gkikae@auth.gr. Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; Natural Products Research Centre of Excellence (NatPro-AUTH), Center for Interdisciplinary Research and Innovation of Aristotle University of Thessaloniki (CIRI-AUTH), 57001 Thessaloniki, Greece. Electronic address: adreana@cheng.auth.gr" |
| Journal Title: | J Chromatogr B Analyt Technol Biomed Life Sci |
| DOI: | 10.1016/j.jchromb.2021.122852 |
| ISSN/ISBN: | 1873-376X (Electronic) 1570-0232 (Linking) |
| Abstract: | "A static headspace gas chromatography - mass spectrometry (HS-GC/MS) method was developed and optimized with the aim to be applied in the analysis of lavender essential oil. To obtain a comprehensive profile of the essential oil, the optimum HS-GC/MS method parameters were selected based on a Design of Experiments (DomicronE) process. Plackett-Burman experimental design was applied by utilizing seven parameters of the HS injection system. Incubation equilibration temperature and time, agitator's vortex speed, post injection dwell time, inlet temperature, split ratio and injection flow rate were screened to select the optimum conditions on the basis of the number and the intensity of the identified compounds. Other parameters, such as sample volume and dilution solvent ratio, were also examined to achieve a comprehensive profile in a chromatographic run of 55 min. With the obtained optimum method, more than 40 volatile compounds were identified in lavender's essential oils from different geographical regions in Greece. The method can be utilized for the quality assessment of lavender's essential oil and provide information on its characteristic aroma and discrimination among species based on the acquired GC-MS profiles" |
| Keywords: | "Gas Chromatography-Mass Spectrometry/*methods Greece Lavandula/*chemistry Linear Models Odorants/analysis *Oils, Volatile/analysis/chemistry/classification *Plant Oils/analysis/chemistry/classification Research Design Design of experiments Essential oils;" |
| Notes: | "MedlineVirgiliou, Christina Zisi, Chrysostomi Kontogiannopoulos, Konstantinos N Nakas, Alexandros Iakovakis, Achilleas Varsamis, Vasilios Gika, Helen G Assimopoulou, Andreana N eng Netherlands 2021/07/19 J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Aug 1; 1179:122852. doi: 10.1016/j.jchromb.2021.122852. Epub 2021 Jul 5" |
