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Proc Natl Acad Sci U S A

Title:		Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing in Saccharomyces cerevisiae
Author(s):		Vasen G; Dunayevich P; Colman-Lerner A;
Address:		"Department of Physiology, Molecular and Cellular Biology, School of Exact and Natural Sciences, University of Buenos Aires (UBA), C1428EGA Buenos Aires, Argentina. Institute of Physiology, Molecular Biology and Neurosciences, National Council of Scientific and Technical Research (IFIBYNE-UBA-CONICET), C1428EGA Buenos Aires, Argentina. Department of Physiology, Molecular and Cellular Biology, School of Exact and Natural Sciences, University of Buenos Aires (UBA), C1428EGA Buenos Aires, Argentina; colman-lerner@fbmc.fcen.uba.ar"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2020
Volume:		20200309
Issue:		12
Page Number:		6580 - 6589
DOI:		10.1073/pnas.1912505117
ISSN/ISBN:		1091-6490 (Electronic) 0027-8424 (Print) 0027-8424 (Linking)
Abstract:		"Polarity decisions are central to many processes, including mitosis and chemotropism. In Saccharomyces cerevisiae, budding and mating projection (MP) formation use an overlapping system of cortical landmarks that converges on the small G protein Cdc42. However, pheromone-gradient sensing must override the Rsr1-dependent internal polarity cues used for budding. Using this model system, we asked what happens when intrinsic and extrinsic spatial cues are not aligned. Is there competition, or collaboration? By live-cell microscopy and microfluidics techniques, we uncovered three previously overlooked features of this signaling system. First, the cytokinesis-associated polarization patch serves as a polarity landmark independently of all known cues. Second, the Rax1-Rax2 complex functions as a pheromone-promoted polarity cue in the distal pole of the cells. Third, internal cues remain active during pheromone-gradient tracking and can interfere with this process, biasing the location of MPs. Yeast defective in internal-cue utilization align significantly better than wild type with artificially generated pheromone gradients"
Keywords:		Cell Cycle *Cell Polarity *Chemotaxis Cytokinesis Mating Factor/*metabolism Membrane Proteins/genetics/metabolism Mutation Saccharomyces cerevisiae/cytology/*metabolism Saccharomyces cerevisiae Proteins/genetics/metabolism Signal Transduction cdc42 GTP-Bi;neuroscience;
Notes:		"MedlineVasen, Gustavo Dunayevich, Paula Colman-Lerner, Alejandro eng R01 GM097479/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2020/03/11 Proc Natl Acad Sci U S A. 2020 Mar 24; 117(12):6580-6589. doi: 10.1073/pnas.1912505117. Epub 2020 Mar 9"