| Title: | Effect of transportation and storage using sorbent tubes of exhaled breath samples on diagnostic accuracy of electronic nose analysis |
| Author(s): | van der Schee MP; Fens N; Brinkman P; Bos LD; Angelo MD; Nijsen TM; Raabe R; Knobel HH; Vink TJ; Sterk PJ; |
| Address: | "Department of Respiratory Medicine, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands. m.p.vanderschee@amc.uva.nl" |
| DOI: | 10.1088/1752-7155/7/1/016002 |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "Many (multi-centre) breath-analysis studies require transport and storage of samples. We aimed to test the effect of transportation and storage using sorbent tubes of exhaled breath samples for diagnostic accuracy of eNose and GC-MS analysis. As a reference standard for diagnostic accuracy, breath samples of asthmatic patients and healthy controls were analysed by three eNose devices. Samples were analysed by GC-MS and eNose after 1, 7 and 14 days of transportation and storage using sorbent tubes. The diagnostic accuracy for eNose and GC-MS after storage was compared to the reference standard. As a validation, the stability was assessed of 15 compounds known to be related to asthma, abundant in breath or related to sampling and analysis. The reference test discriminated asthma and healthy controls with a median AUC (range) of 0.77 (0.72-0.76). Similar accuracies were achieved at t1 (AUC eNose 0.78; GC-MS 0.84), t7 (AUC eNose 0.76; GC-MS 0.79) and t14 (AUC eNose 0.83; GC-MS 0.84). The GC-MS analysis of compounds showed an adequate stability for all 15 compounds during the 14 day period. Short-term transportation and storage using sorbent tubes of breath samples does not influence the diagnostic accuracy for discrimination between asthma and health by eNose and GC-MS" |
| Keywords: | Adult Asthma/metabolism Breath Tests/*instrumentation Case-Control Studies Cross-Sectional Studies Exhalation Female Gas Chromatography-Mass Spectrometry Humans Male Middle Aged *Specimen Handling/instrumentation Volatile Organic Compounds/metabolism; |
| Notes: | "Medlinevan der Schee, M P Fens, N Brinkman, P Bos, L D J Angelo, M D Nijsen, T M E Raabe, R Knobel, H H Vink, T J Sterk, P J eng Research Support, Non-U.S. Gov't England 2012/12/22 J Breath Res. 2013 Mar; 7(1):016002. doi: 10.1088/1752-7155/7/1/016002. Epub 2012 Dec 21" |
