| Title: | The effect of a paced auditory serial addition test (PASAT) intervention on the profile of volatile organic compounds in human breath: a pilot study |
| Author(s): | Turner MA; Bandelow S; Edwards L; Patel P; Martin HJ; Wilson ID; Thomas CL; |
| Address: | "Centre for Analytical Science, Loughborough University, Leicestershire, UK" |
| DOI: | 10.1088/1752-7155/7/1/017102 |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "This study sought to identify if detectable changes in human breath profiles may be observed following a psychological intervention designed to induce stress, a paced auditory serial addition test (PASAT). Breath samples were collected from 22 participants (10 male and 12 female) following a double cross-over randomized design with two experimental interventions. One intervention required participants to listen to classical music chosen to be neutral. The other intervention required participants to undertake a PASAT that induced cardiovascular responses consistent with acute stress. Both interventions also involved two sequences of cognitive function tests. Blood-pressure and heart-rate were recorded throughout each intervention and distal breath samples were collected onto Tenax(R) TA/Carbograph 1 thermal desorption tubes, using an adaptive breath sampler. Samples were collected before and after the PASAT. Breath samples were analysed by thermal desorption gas chromatography-mass spectrometry. Data registration using retention indexing and peak deconvolution followed by partial least-squares discriminant analysis identified six stress sensitive compounds. A principal components analysis model based on these components generated a model that predicted post-PASAT versus post-neutral intervention samples with a sensitivity of 83.3% and a selectivity of 91.6% for females, compared to 100% sensitivity and 90% selectivity for males. Of the six compounds indole, 2-hydroxy-1-phenylethanone, benzaldehyde, and 2-ethylhexan-1-ol were identified on the basis of mass spectral, retention indexing and confirmation against pure standards. 2-methylpentadecane was tentatively identified from mass spectral and retention indexing, whilst one component has yet to be assigned, although the mass spectrum is indicative of a terpene. Indole and 2-methylpentadecane concentrations increased in response to the PASAT intervention, while the other compounds reduced in their abundance in human breath, possibly as a result of ventilation effects" |
| Keywords: | *Acoustic Stimulation Blood Pressure *Breath Tests Cross-Over Studies Exhalation Female Gas Chromatography-Mass Spectrometry Heart Rate Humans Male *Neuropsychological Tests Pilot Projects Principal Component Analysis Sensitivity and Specificity Volatile; |
| Notes: | "MedlineTurner, M A Bandelow, S Edwards, L Patel, P Martin, H J Wilson, I D Thomas, C L P eng Randomized Controlled Trial Research Support, Non-U.S. Gov't England 2013/03/01 J Breath Res. 2013 Mar; 7(1):017102. doi: 10.1088/1752-7155/7/1/017102. Epub 2013 Feb 27" |
