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Nat Prod Commun

Title:		"Antibacterial activity and comparison of the volatile constituents obtained by several extraction methods from the flowers, stems and leaves of Astrodaucus orientalis"
Author(s):		Torabbeigi M; Azar PA; Sharifan A; Meibodi ZA;
Address:		"Faculty of Health, Safety and Environment, Shahid Beheshti University, M.C., P.O. Box 16858-116, Tehran, Iran. torabbeigi@gmail.com"
Journal Title:		Nat Prod Commun
Year:		2012
Volume:		7
Issue:		2
Page Number:		249 - 252
DOI:
ISSN/ISBN:		1934-578X (Print) 1555-9475 (Linking)
Abstract:		"The oils of the flowers, stems and leaves of Astrodaucus orientalis L. were separately extracted using hydrodistillation (HD), head-space solid-phase microextraction (HS-SPME) and microwave assisted head-space solid-phase microextraction (MA-HS-SPME). The volatile constituents were analyzed by GC and GC/MS. Temperature and time of extraction, microwave power and exposure time of extraction were optimized. Polydimethylsiloxane (PDMS) fiber was used as the solid phase for SPME methods. The main constituents of the flower, stem and leaf oils isolated by HD, HS-SPME and MA-HS-SPME are as follows, respectively: beta-pinene (20.5%, 13.9% and 30.4%), alpha-thujene (8.7%, 16.2% and 10.9%) and alpha-pinene (7.6%, 14.3% and 10.9%) for the flowers, sabinene (11.8%, 32.3% and 11.8%), alpha-pinene (8.7%, 28.8% and 6.1%) and p-mycrene (2.5%, 12% and 8.5%) for the stem, and alpha-pinene (9.4%, 37.1% and 22.5%), sabinene (13.5%, 26.3% and 23.5%), beta-pinene (6.3%, 9.8% and 10%) and p-mycrene (3.2%, 2.5% and 15.6%) for the leaf. The minimum inhibitory concentrations (MIC) were determined for all essential oils obtained by HD against both Gram-positive (Bacillus subtilis) and Gram-negative (Escherichia coli) bacteria using the agar dilution method. These oils showed the good activities against the both bacteria (0.5 - 1.5 mg/mL)"
Keywords:		Anti-Bacterial Agents/chemistry/*pharmacology Apiaceae/*chemistry Chemical Fractionation/methods Flowers/*chemistry Microwaves Plant Leaves/*chemistry Plant Stems/*chemistry Volatile Organic Compounds/chemistry/*pharmacology;
Notes:		"MedlineTorabbeigi, Marzieh Azar, Parviz Abroomand Sharifan, Anousheh Meibodi, Zahra Aghaei eng 2012/04/06 Nat Prod Commun. 2012 Feb; 7(2):249-52"