| Title: | "Levels of cytokines in broncho-alveolar lavage fluid, but not in plasma, are associated with levels of markers of lipid peroxidation in breath of ventilated ICU patients" |
| Author(s): | Boshuizen M; Leopold JH; Zakharkina T; Knobel HH; Weda H; Nijsen TM; Vink TJ; Sterk PJ; Schultz MJ; Bos LD; consortium M; |
| Address: | "Intensive Care Unit, Academic Medical Center-University of Amsterdam, The Netherlands" |
| DOI: | 10.1088/1752-7155/9/3/036010 |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "Alkanes and alkenes in the breath are produced through fatty acid peroxidation, which is initialized by reactive oxygen species. Inflammation is an important cause and effect of reactive oxygen species. We aimed to evaluate the association between fatty acid peroxidation products and inflammation of the alveolar and systemic compartment in ventilated intensive care unit (ICU) patients.Volatile organic compounds were measured by gas chromatography and mass spectrometry in the breath of newly ventilated ICU patients within 24 h after ICU admission. Cytokines were measured in non-directed bronchial lavage fluid (NBL) and plasma by cytometric bead array. Correlation coefficients were calculated and presented in heatmaps.93 patients were included. Peroxidation products in exhaled breath were not associated with markers of inflammation in plasma, but were correlated with those in NBL. IL-6, IL-8, IL-1beta and TNF-alpha concentration in NBL showed inverse correlation coefficients with the peroxidation products of fatty acids. Furthermore, NBL IL-10, IL-13, GM-CSF and IFNgamma demonstrated positive associations with breath alkanes and alkenes. Correlation coefficients for NBL cytokines were high regarding peroxidation products of n-6, n-7 and particularly in n-9 fatty acids.Levels of lipid peroxidation products in the breath of ventilated ICU patients are associated with levels of inflammatory markers in NBL, but not in plasma. Alkanes and alkenes in breath seems to be associated with an anti-inflammatory, rather than a pro-inflammatory state in the alveoli" |
| Keywords: | Adult Aged Biomarkers/analysis Breath Tests/methods Bronchoalveolar Lavage Fluid/*chemistry Critical Care Cytokines/*analysis/blood Exhalation Female Humans Intensive Care Units Lipid Peroxidation/*physiology Lung/physiopathology Male Middle Aged Reactive; |
| Notes: | "MedlineBoshuizen, Margit Leopold, Jan Hendrik Zakharkina, Tetyana Knobel, Hugo H Weda, Hans Nijsen, Tamara M E Vink, Teunis J Sterk, Peter J Schultz, Marcus J Bos, Lieuwe D J eng England 2015/09/04 J Breath Res. 2015 Sep 3; 9(3):036010. doi: 10.1088/1752-7155/9/3/036010" |
